PURPOSE: Investigation of the oxygenation status of organotypic multicellular spheroids (OMS) and their response to irradiation. MATERIALS AND METHODS: Tumour specimens of glioblastoma multiforme patients (n = 16) were initiated as OMS. Following 20 Gy gamma-irradiation, the cell migratory capacity was evaluated. Spheroid oxygenation was determined by micro-electrode pO2 measurements and pimonidazole immunostaining. Spheroids prepared from established human glioma cell lines were used as a reference. RESULTS: Irradiation inhibited spheroid outgrowth by 12 to 88% relative to the non-irradiated controls. A large interpatient variation was noticed. Oxygen measurements revealed a gradual decrease in pO2 level from the periphery to the core of the spheroids, but the pO2 values remained within an oxygenated range. However, in the cell line spheroids an intermediate layer of hypoxia surrounding the central core was observed. CONCLUSION: Cell line spheroids with a hypoxic cell fraction and well-oxygenated OMS both show high resistance to irradiation, indicating that hypoxia may not be the biological factor determining the radioresistance of glioma spheroids in vitro.
PURPOSE: Investigation of the oxygenation status of organotypic multicellular spheroids (OMS) and their response to irradiation. MATERIALS AND METHODS: Tumour specimens of glioblastoma multiformepatients (n = 16) were initiated as OMS. Following 20 Gy gamma-irradiation, the cell migratory capacity was evaluated. Spheroid oxygenation was determined by micro-electrode pO2 measurements and pimonidazole immunostaining. Spheroids prepared from established humanglioma cell lines were used as a reference. RESULTS: Irradiation inhibited spheroid outgrowth by 12 to 88% relative to the non-irradiated controls. A large interpatient variation was noticed. Oxygen measurements revealed a gradual decrease in pO2 level from the periphery to the core of the spheroids, but the pO2 values remained within an oxygenated range. However, in the cell line spheroids an intermediate layer of hypoxia surrounding the central core was observed. CONCLUSION: Cell line spheroids with a hypoxic cell fraction and well-oxygenated OMS both show high resistance to irradiation, indicating that hypoxia may not be the biological factor determining the radioresistance of glioma spheroids in vitro.
Authors: Hari R Kumar; Xiaoling Zhong; Derek J Hoelz; Frederick J Rescorla; Robert J Hickey; Linda H Malkas; John A Sandoval Journal: Pediatr Surg Int Date: 2008-09-17 Impact factor: 1.827
Authors: Fabian Fehlauer; Martina Muench; Dirk Rades; Lukas J A Stalpers; Sieger Leenstra; Paul van der Valk; Ben Slotman; Ernst J Smid; Peter Sminia Journal: J Cancer Res Clin Oncol Date: 2005-11-01 Impact factor: 4.553
Authors: Charlotte Aaberg-Jessen; Annette Nørregaard; Karina Christensen; Christian B Pedersen; Claus Andersen; Bjarne W Kristensen Journal: Int J Clin Exp Pathol Date: 2013-03-15