BACKGROUND: We studied the effects of all-trans retinoic acid (ATRA) and Bone morphogenetic protein-6 (BMP-60 on neuronal differentiation of IMR-32 cells. MATERIALS AND METHODS: Immunostaining, Western blotting and MTT assays were used to characterize the survival and neuronal phenotype of IMR-32 cells differentiated by ATRA +/- BMP-6. RESULTS & CONCLUSION: ATRA, BMP-6 and ATRA + BMP-6 treatments each induced dopaminergic neuronal differentiation of the IMR-32 cells as judged by expression of GAP-43, tyrosine hydroxylase and microtubule-associated protein 2ab. ATRA alone induced strong biochemical differentiation, whereas ATRA + BMP-6 treatment synergistically enhanced neurite outgrowth and morphological maturation. When compared with undifferentiated controls, ATRA induced Bcl-2 expression, while loss of Bax expression was observed only in cells differentiated by ATRA + BMP-6. The high Bcl-2/Bax ratio in cells differentiated by ATRA and ATRA + BMP-6, respectively, correlated with the survival of these cells in serum-free media. Upon re-exposure to fresh serum lacking factors, only cells differentiated by ATRA + BMP-6 were unable to resume cell division. In concordance with their morphological maturation, these cells were terminally differentiated when compared with cells differentiated by separate treatment with ATRA or BMP-6.
BACKGROUND: We studied the effects of all-trans retinoic acid (ATRA) and Bone morphogenetic protein-6 (BMP-60 on neuronal differentiation of IMR-32 cells. MATERIALS AND METHODS: Immunostaining, Western blotting and MTT assays were used to characterize the survival and neuronal phenotype of IMR-32 cells differentiated by ATRA +/- BMP-6. RESULTS & CONCLUSION:ATRA, BMP-6 and ATRA + BMP-6 treatments each induced dopaminergic neuronal differentiation of the IMR-32 cells as judged by expression of GAP-43, tyrosine hydroxylase and microtubule-associated protein 2ab. ATRA alone induced strong biochemical differentiation, whereas ATRA + BMP-6 treatment synergistically enhanced neurite outgrowth and morphological maturation. When compared with undifferentiated controls, ATRA induced Bcl-2 expression, while loss of Bax expression was observed only in cells differentiated by ATRA + BMP-6. The high Bcl-2/Bax ratio in cells differentiated by ATRA and ATRA + BMP-6, respectively, correlated with the survival of these cells in serum-free media. Upon re-exposure to fresh serum lacking factors, only cells differentiated by ATRA + BMP-6 were unable to resume cell division. In concordance with their morphological maturation, these cells were terminally differentiated when compared with cells differentiated by separate treatment with ATRA or BMP-6.
Authors: R M Parry; W Jones; T H Stokes; J H Phan; R A Moffitt; H Fang; L Shi; A Oberthuer; M Fischer; W Tong; M D Wang Journal: Pharmacogenomics J Date: 2010-08 Impact factor: 3.550
Authors: C Vadasz; J F Smiley; K Figarsky; M Saito; R Toth; B M Gyetvai; M Oros; K K Kovacs; P Mohan; R Wang Journal: Neuroscience Date: 2007-07-17 Impact factor: 3.590
Authors: Sidong Huang; Jamila Laoukili; Mirjam T Epping; Jan Koster; Michael Hölzel; Bart A Westerman; Wouter Nijkamp; Akiko Hata; Shahab Asgharzadeh; Robert C Seeger; Rogier Versteeg; Roderick L Beijersbergen; René Bernards Journal: Cancer Cell Date: 2009-04-07 Impact factor: 31.743
Authors: Amnah M Alshangiti; Sean L Wyatt; Erin McCarthy; Louise M Collins; Shane V Hegarty; Aideen M Sullivan; Gerard W O'Keeffe Journal: Neuronal Signal Date: 2020-04-23