| Literature DB >> 1281794 |
T Zink1, A Ross, D Ambrosius, R Rudolph, T A Holak.
Abstract
Recombinant 15N-, 13C-labeled human granulocyte colony-stimulating factor (rh-metG-CSF) has been studied by 2D and 3D NMR using uniformly labeled protein as well as residue-specific 15N-labeled samples. Assignment of the 1H, 15N backbone, and 60% 1H sidechain resonances has enabled the determination of the secondary structure of the protein. The secondary structure is dominated by alpha-helical regions with four stretches of helices between residues 11-41, 71-95, 102-124 and 144-170.Entities:
Mesh：
Substances：
Year: 1992 PMID： 1281794 DOI： 10.1016/0014-5793(92)81521-m
Source DB: PubMed Journal: FEBS Lett ISSN： 0014-5793 Impact factor: 4.124