Corticotropin releasing factor and galanin-containing neurons projecting to the median eminence of the rat.

To identify corticotropin releasing factor (CRF)- and galanin (GAL)-containing neurons projecting to the median eminence, a retrograde tracing method with True blue was combined with a double-staining method. It was demonstrated that some True blue-labeled CRF neurons in the paraventricular nucleus were also immunoreactive for GAL. These findings support the hypothesis that GAL and CRF are simultaneously involved in regulation of ACTH secretion from the anterior pituitary.