M I Khan1, A A Fadl, K S Venkitanarayanan. 1. Department of Pathobiology, University of Connecticut, Storrs, CT 06269, USA. mazhar.khan@uconn.edu
Abstract
AIMS: To evaluate the ability of Salmonella enterica ser. Enteritidis outer membrane proteins (OMPs) of 75.6 and 82.3 kDa to inhibit or reduce in vivo colonization of S. Enteritidis on intestinal mucosa in chickens. METHODS AND RESULTS: Nine-week-old specific-pathogen-free chickens were subcutaneously immunized with 75.6 or 82.3 kDa protein, and challenged with a virulent strain of S. Enteritidis. Chickens were killed, and portions of small intestine and caecum were removed at necropsy. The population of S. Enteritidis attached to chicken intestinal mucosa was determined. The population of S. Enteritidis recovered from the small intestine and caecum of chickens immunized with 75.6 or 82.3 kDa protein was significantly (P < 0.05) lower than that recovered from the control birds. CONCLUSIONS: Salmonella Enteritidis OMPs 75.6 kDa and 82.3 kDa were effective in reducing colonization of S. Enteritidis on intestinal mucosa in chickens. SIGNIFICANCE AND IMPACT OF THE STUDY: Salmonella Enteritidis OMPs 75.6 or 82.3 kDa could be used as potential vaccines to reduce S. Enteritidis colonization in chickens.
AIMS: To evaluate the ability of Salmonella enterica ser. Enteritidis outer membrane proteins (OMPs) of 75.6 and 82.3 kDa to inhibit or reduce in vivo colonization of S. Enteritidis on intestinal mucosa in chickens. METHODS AND RESULTS: Nine-week-old specific-pathogen-free chickens were subcutaneously immunized with 75.6 or 82.3 kDa protein, and challenged with a virulent strain of S. Enteritidis. Chickens were killed, and portions of small intestine and caecum were removed at necropsy. The population of S. Enteritidis attached to chicken intestinal mucosa was determined. The population of S. Enteritidis recovered from the small intestine and caecum of chickens immunized with 75.6 or 82.3 kDa protein was significantly (P < 0.05) lower than that recovered from the control birds. CONCLUSIONS:Salmonella Enteritidis OMPs 75.6 kDa and 82.3 kDa were effective in reducing colonization of S. Enteritidis on intestinal mucosa in chickens. SIGNIFICANCE AND IMPACT OF THE STUDY: Salmonella Enteritidis OMPs 75.6 or 82.3 kDa could be used as potential vaccines to reduce S. Enteritidis colonization in chickens.
Authors: Anup Kollanoor-Johny; Tyler Mattson; Sangeetha Ananda Baskaran; Mary Anne Amalaradjou; Sankhiros Babapoor; Benjamin March; Satyender Valipe; Michael Darre; Thomas Hoagland; David Schreiber; Mazhar I Khan; Ann Donoghue; Dan Donoghue; Kumar Venkitanarayanan Journal: Appl Environ Microbiol Date: 2012-02-10 Impact factor: 4.792
Authors: Shakeeba Waseh; Pejman Hanifi-Moghaddam; Russell Coleman; Michael Masotti; Shannon Ryan; Mary Foss; Roger MacKenzie; Matthew Henry; Christine M Szymanski; Jamshid Tanha Journal: PLoS One Date: 2010-11-22 Impact factor: 3.240