AIMS: Mutans streptococci such as Streptococcus mutans and Streptococcus sobrinus have been implicated in human dental caries. In an attempt to develop a rapid and sensitive method for detecting Strep. mutans and Strep. sobrinus in dental plaque, a nested PCR amplification based on the 16S rRNA gene was employed. METHODS AND RESULTS: A universal set of PCR primers for bacterial 16S rRNA gene was introduced for the first PCR, and then two sets of primers specific for the 16S rRNA gene sequences of either Strep. mutans or Strep. sobrinus were used for the second PCR. Eighteen plaque samples were analyzed, and a nested PCR was shown to be more sensitive for detecting Strep. mutans and Strep. sobrinus than direct PCR. CONCLUSIONS, SIGNIFICANCE AND IMPACT OF THE STUDY: The 16S rRNA gene-based nested PCR method is a rapid and sensitive method for the detection of mutans streptococci, and may also be suitable for carrying out large-scale studies on the cariogenicity of mutans streptococci.
AIMS: Mutans streptococci such as Streptococcus mutans and Streptococcus sobrinus have been implicated in human dental caries. In an attempt to develop a rapid and sensitive method for detecting Strep. mutans and Strep. sobrinus in dental plaque, a nested PCR amplification based on the 16S rRNA gene was employed. METHODS AND RESULTS: A universal set of PCR primers for bacterial 16S rRNA gene was introduced for the first PCR, and then two sets of primers specific for the 16S rRNA gene sequences of either Strep. mutans or Strep. sobrinus were used for the second PCR. Eighteen plaque samples were analyzed, and a nested PCR was shown to be more sensitive for detecting Strep. mutans and Strep. sobrinus than direct PCR. CONCLUSIONS, SIGNIFICANCE AND IMPACT OF THE STUDY: The 16S rRNA gene-based nested PCR method is a rapid and sensitive method for the detection of mutans streptococci, and may also be suitable for carrying out large-scale studies on the cariogenicity of mutans streptococci.
Authors: P Saraithong; K Pattanaporn; Z Chen; S Khongkhunthian; P Laohapensang; N Chhun; W Pattanaporn; H Y Gaw; Y Li Journal: Clin Oral Investig Date: 2015-03-11 Impact factor: 3.573
Authors: C A Palmer; R Kent; C Y Loo; C V Hughes; E Stutius; N Pradhan; M Dahlan; E Kanasi; S S Arevalo Vasquez; A C R Tanner Journal: J Dent Res Date: 2010-09-21 Impact factor: 6.116
Authors: Elizabeth A Palmer; Truman Nielsen; Patricia Peirano; Anna T Nguyen; Alex Vo; Aivan Nguyen; Stephen Jackson; Tyler Finlayson; Rebecca Sauerwein; Katie Marsh; Issac Edwards; Beth Wilmot; John Engle; John Peterson; Tom Maier; Curtis A Machida Journal: Pediatr Dent Date: 2012 Mar-Apr Impact factor: 1.874
Authors: Richard M Gibson; Ashley M Meyer; Dane Winner; John Archer; Felix Feyertag; Ezequiel Ruiz-Mateos; Manuel Leal; David L Robertson; Christine L Schmotzer; Miguel E Quiñones-Mateu Journal: Antimicrob Agents Chemother Date: 2014-01-27 Impact factor: 5.191
Authors: Lingyang Tian; Takuichi Sato; Kousuke Niwa; Mitsuo Kawase; Anne C R Tanner; Nobuhiro Takahashi Journal: Biomed Res Int Date: 2014-11-17 Impact factor: 3.411