OBJECTIVE: To characterise in vitro engineered cartilaginous constructs made employing a novel static, scaffold-free and closed chamber system. DESIGN: Chondrocytes derived from slaughter age pigs (3-6 months) were seeded at high density (20 x 10(6)) into cylindrical chambers (1.0 x 0.5cm) and were maintained between an upper and a lower membrane (100 kDa) for 21 days and subsequently cultured in open culture for 7 additional days. RESULTS: Viable constructs produced were approximately 10 mmx2mm in size and were stable enough to be handled by surgical pincers. Histology and electron microscopy evaluations revealed a neo-cartilage structure of high cell density with a comprehensive extracellular matrix. Predominately collagen type II and negligible amounts of collagen types I and X were detected using RT-PCR and SDS-PAGE analyses. CONCLUSIONS: In this study, we provide evidence of a scaffold-free system that can produce immature hyaline-like cartilaginous constructs suitable for in vivo implantation, or that may be useful for in vitro studies of events related to the process of chondrogenesis.
OBJECTIVE: To characterise in vitro engineered cartilaginous constructs made employing a novel static, scaffold-free and closed chamber system. DESIGN: Chondrocytes derived from slaughter age pigs (3-6 months) were seeded at high density (20 x 10(6)) into cylindrical chambers (1.0 x 0.5cm) and were maintained between an upper and a lower membrane (100 kDa) for 21 days and subsequently cultured in open culture for 7 additional days. RESULTS: Viable constructs produced were approximately 10 mmx2mm in size and were stable enough to be handled by surgical pincers. Histology and electron microscopy evaluations revealed a neo-cartilage structure of high cell density with a comprehensive extracellular matrix. Predominately collagen type II and negligible amounts of collagen types I and X were detected using RT-PCR and SDS-PAGE analyses. CONCLUSIONS: In this study, we provide evidence of a scaffold-free system that can produce immature hyaline-like cartilaginous constructs suitable for in vivo implantation, or that may be useful for in vitro studies of events related to the process of chondrogenesis.
Authors: Matteo Centola; Franca Abbruzzese; Celeste Scotti; Andrea Barbero; Gianluca Vadalà; Vincenzo Denaro; Ivan Martin; Marcella Trombetta; Alberto Rainer; Anna Marsano Journal: Tissue Eng Part A Date: 2013-05-30 Impact factor: 3.845
Authors: Simon Ströbel; Marko Loparic; David Wendt; Andreas D Schenk; Christian Candrian; Raija L P Lindberg; Florina Moldovan; Andrea Barbero; Ivan Martin Journal: Arthritis Res Ther Date: 2010-03-02 Impact factor: 5.156
Authors: Claire Mennan; John Garcia; Helen McCarthy; Sharon Owen; Jade Perry; Karina Wright; Robin Banerjee; James B Richardson; Sally Roberts Journal: Cartilage Date: 2018-04-19 Impact factor: 4.634