AIM: To explore the role and significance of costimulatory molecules B7H1,B7H2 and ICOS within tissues of human gastric carcinoma and the possible mechanisms in tumor escape. METHODS: mRNA expressions of costimulatory molecules including B7H1,B7H2,ICOS and B7-1 in tissues of human gastric carcinoma were investigated by in situ hybridization using digoxigenin-labeled oligonucleotide-probes. The tissue of chronic gastric ulcer was used as a control. All data were analyzed by SPSS statistic software. RESULTS: At the site of gastric carcinoma, mRNA expression levels of B7H1, B7H2 and ICOS were much higher than that of B7-1. Their mRNA positive expression indexes were 0.512+/-0.333, 0.812+/-0.454, 0.702+/-0.359 and 0.293+/-0.253, respectively. The positively stained cells were mainly tumor infiltrating lymphocytes (TILs), and some tumor cells. The difference between them was greatly significant P<0.005. The mRNA expression levels of four molecules were not correlated to the pathological grade and matastasis of gastric carcinoma. CONCLUSION: ICOS-B7H costimulatory pathway may be predominant at the site of gastric carcinoma. B7-1mRNA might be the basis of ICOS-B7H interaction. ICOS-B7H interaction induces the production of IL-10 which inhibits the antitumor immune responses. Therefore, it is supposed that ICOS-B7H costimulatory pathway may be involved in the negative regulation of cell-mediated immune responses.
AIM: To explore the role and significance of costimulatory molecules B7H1,B7H2 and ICOS within tissues of humangastric carcinoma and the possible mechanisms in tumor escape. METHODS: mRNA expressions of costimulatory molecules including B7H1,B7H2,ICOS and B7-1 in tissues of humangastric carcinoma were investigated by in situ hybridization using digoxigenin-labeled oligonucleotide-probes. The tissue of chronic gastric ulcer was used as a control. All data were analyzed by SPSS statistic software. RESULTS: At the site of gastric carcinoma, mRNA expression levels of B7H1, B7H2 and ICOS were much higher than that of B7-1. Their mRNA positive expression indexes were 0.512+/-0.333, 0.812+/-0.454, 0.702+/-0.359 and 0.293+/-0.253, respectively. The positively stained cells were mainly tumor infiltrating lymphocytes (TILs), and some tumor cells. The difference between them was greatly significant P<0.005. The mRNA expression levels of four molecules were not correlated to the pathological grade and matastasis of gastric carcinoma. CONCLUSION:ICOS-B7H costimulatory pathway may be predominant at the site of gastric carcinoma. B7-1mRNA might be the basis of ICOS-B7H interaction. ICOS-B7H interaction induces the production of IL-10 which inhibits the antitumor immune responses. Therefore, it is supposed that ICOS-B7H costimulatory pathway may be involved in the negative regulation of cell-mediated immune responses.
Authors: A Tafuri; A Shahinian; F Bladt; S K Yoshinaga; M Jordana; A Wakeham; L M Boucher; D Bouchard; V S Chan; G Duncan; B Odermatt; A Ho; A Itie; T Horan; J S Whoriskey; T Pawson; J M Penninger; P S Ohashi; T W Mak Journal: Nature Date: 2001-01-04 Impact factor: 49.962
Authors: S K Yoshinaga; J S Whoriskey; S D Khare; U Sarmiento; J Guo; T Horan; G Shih; M Zhang; M A Coccia; T Kohno; A Tafuri-Bladt; D Brankow; P Campbell; D Chang; L Chiu; T Dai; G Duncan; G S Elliott; A Hui; S M McCabe; S Scully; A Shahinian; C L Shaklee; G Van; T W Mak; G Senaldi Journal: Nature Date: 1999-12-16 Impact factor: 49.962
Authors: S Ikemizu; R J Gilbert; J A Fennelly; A V Collins; K Harlos; E Y Jones; D I Stuart; S J Davis Journal: Immunity Date: 2000-01 Impact factor: 31.745
Authors: A J McAdam; T T Chang; A E Lumelsky; E A Greenfield; V A Boussiotis; J S Duke-Cohan; T Chernova; N Malenkovich; C Jabs; V K Kuchroo; V Ling; M Collins; A H Sharpe; G J Freeman Journal: J Immunol Date: 2000-11-01 Impact factor: 5.422
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