Literature DB >> 12798994

Isolation of high molecular weight DNA from soil for cloning into BAC vectors.

Andrew E Berry1, Claudia Chiocchini, Tina Selby, Margherita Sosio, Elizabeth M H Wellington.   

Abstract

Isolation of high molecular weight DNA fragments from soil, in excess of 1 Mb, and of sufficient quality for cloning into an Escherichia coli-streptomycete artificial chromosome vector is described. The combination of indirect extraction of cells, using a nycodenz extraction technique, followed by lysis of biomass immobilised in agarose plugs, allowed fragments in excess of 1 Mb to be purified.

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Year:  2003        PMID： 12798994     DOI： 10.1016/S0378-1097(03)00248-9

Source DB:  PubMed          Journal:  FEMS Microbiol Lett        ISSN： 0378-1097            Impact factor:   2.742


  17 in total

1.  Current evidence for human yersiniosis in Ireland.

Authors:  T Ringwood; B P Murphy; N Drummond; J F Buckley; A P Coveney; H P Redmond; J P Power; S Fanning; M B Prentice
Journal:  Eur J Clin Microbiol Infect Dis       Date:  2012-06-03       Impact factor: 3.267

2.  Size Does Matter: Application-driven Approaches for Soil Metagenomics.

Authors:  Kavita S Kakirde; Larissa C Parsley; Mark R Liles
Journal:  Soil Biol Biochem       Date:  2010-11-01       Impact factor: 7.609

Review 3.  Metagenomics: application of genomics to uncultured microorganisms.

Authors:  Jo Handelsman
Journal:  Microbiol Mol Biol Rev       Date:  2004-12       Impact factor: 11.056

4.  Environmental whole-genome amplification to access microbial populations in contaminated sediments.

Authors:  Carl B Abulencia; Denise L Wyborski; Joseph A Garcia; Mircea Podar; Wenqiong Chen; Sherman H Chang; Hwai W Chang; David Watson; Eoin L Brodie; Terry C Hazen; Martin Keller
Journal:  Appl Environ Microbiol       Date:  2006-05       Impact factor: 4.792

Review 5.  Microarray applications in microbial ecology research.

Authors:  T J Gentry; G S Wickham; C W Schadt; Z He; J Zhou
Journal:  Microb Ecol       Date:  2006-08-08       Impact factor: 4.552

6.  Targeted access to the genomes of low-abundance organisms in complex microbial communities.

Authors:  Mircea Podar; Carl B Abulencia; Marion Walcher; Don Hutchison; Karsten Zengler; Joseph A Garcia; Trevin Holland; David Cotton; Loren Hauser; Martin Keller
Journal:  Appl Environ Microbiol       Date:  2007-03-16       Impact factor: 4.792

7.  Recovery, purification, and cloning of high-molecular-weight DNA from soil microorganisms.

Authors:  Mark R Liles; Lynn L Williamson; Jitsupang Rodbumrer; Vigdis Torsvik; Robert M Goodman; Jo Handelsman
Journal:  Appl Environ Microbiol       Date:  2008-03-21       Impact factor: 4.792

8.  Isolation of high molecular weight DNA from marine sponge bacteria for BAC library construction.

Authors:  Yongchang Ouyang; Shikun Dai; Lianwu Xie; M S Ravi Kumar; Wei Sun; Huimin Sun; Danling Tang; Xiang Li
Journal:  Mar Biotechnol (NY)       Date:  2009-08-15       Impact factor: 3.619

9.  An improved method for extracting bacteria from soil for high molecular weight DNA recovery and BAC library construction.

Authors:  Juan Liu; Jingquan Li; Li Feng; Hui Cao; Zhongli Cui
Journal:  J Microbiol       Date:  2011-01-09       Impact factor: 3.422

10.  Effect of the earthworms Lumbricus terrestris and Aporrectodea caliginosa on bacterial diversity in soil.

Authors:  Taras Y Nechitaylo; Michail M Yakimov; Miguel Godinho; Kenneth N Timmis; Elena Belogolova; Boris A Byzov; Alexander V Kurakov; David L Jones; Peter N Golyshin
Journal:  Microb Ecol       Date:  2009-11-04       Impact factor: 4.552
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