Literature DB >> 12798314

A low background high-throughput screening (HTS) fluorescence assay for lipases and esterases using acyloxymethylethers of umbelliferone.

Emmanuel Leroy1, Nicolas Bensel, Jean Louis Reymond.   

Abstract

Esters and acyloxymethyl ethers of umbelliferone were evaluated as fluorogenic substrates for lipases and esterases with respect to stability and resistance to non-specific hydrolysis. Isobutyryloxymethyl ether 1c and pivaloxymethyl ether 1d were found to be optimal substrates for enzyme assays, particularly with respect to HTS applications.

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Year:  2003        PMID： 12798314     DOI： 10.1016/s0960-894x(03)00377-9

Source DB:  PubMed          Journal:  Bioorg Med Chem Lett        ISSN： 0960-894X            Impact factor:   2.823


  7 in total

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Authors:  Luke D Lavis; Tzu-Yuan Chao; Ronald T Raines
Journal:  Chembiochem       Date:  2006-08       Impact factor: 3.164

2.  Fluorogenic structure activity library pinpoints molecular variations in substrate specificity of structurally homologous esterases.

Authors:  Alex White; Andrew Koelper; Arielle Russell; Erik M Larsen; Charles Kim; Luke D Lavis; Geoffrey C Hoops; R Jeremy Johnson
Journal:  J Biol Chem       Date:  2018-07-13       Impact factor: 5.157

3.  Profiling Esterases in Mycobacterium tuberculosis Using Far-Red Fluorogenic Substrates.

Authors:  Katie R Tallman; Samantha R Levine; Kimberly E Beatty
Journal:  ACS Chem Biol       Date:  2016-05-23       Impact factor: 5.100

4.  Far-red fluorogenic probes for esterase and lipase detection.

Authors:  Katie R Tallman; Kimberly E Beatty
Journal:  Chembiochem       Date:  2014-12-02       Impact factor: 3.164

5.  Synthesis and utility of fluorogenic acetoxymethyl ethers.

Authors:  Luke D Lavis; Tzu-Yuan Chao; Ronald T Raines
Journal:  Chem Sci       Date:  2011-01-01       Impact factor: 9.825

6.  7-Azidomethoxy-coumarins as profluorophores for templated nucleic acid detection.

Authors:  Raphael M Franzini; Eric T Kool
Journal:  Chembiochem       Date:  2008-12-15       Impact factor: 3.164

7.  Trimethyl lock: a stable chromogenic substrate for esterases.

Authors:  Michael N Levine; Luke D Lavis; Ronald T Raines
Journal:  Molecules       Date:  2008-01-31       Impact factor: 4.411
  7 in total

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