Literature DB >> 12798020

Whole genome fingerprinting and genotyping of multiple drug resistant (MDR) isolates of Pseudomonas aeruginosa from endophthalmitis patients in India.

Niyaz Ahmed1, Abhijit Bal, Aleem A Khan, Mahfooz Alam, Anju Kagal, Vidya Arjunwadkar, Amarsingh Rajput, Ahmed A Majeed, Syed Asad Rahman, Sharmistha Banerjee, Suvarna Joshi, Renu Bharadwaj.   

Abstract

Genome sequence-based fluorescent amplified-fragment length polymorphism (FAFLP) analysis was investigated for fingerprinting and subtyping 42 multiple drug resistant (MDR) isolates of Pseudomonas aeruginosa from post-surgical endophthalmitis. The FAFLP profiles derived from EcoRI/MseI restricted fragments differentiated clinical isolates and were found to be extremely reproducible. Seventeen different amplification patterns (amplitypes) were observed for all the 42 isolates from 11 different patients. Also, we were able to genotype the isolates based on the differential amplification of a total of 31 FAFLP markers representing genomic fragments from the P. aeruginosa chromosome. This data appears to provide clues to the genetic diversity of endopthalmitis associated strains and could be converted into digitised fingerprints suitable for electronic manipulations and archiving.

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Year:  2002        PMID： 12798020     DOI： 10.1016/s1567-1348(02)00030-8

Source DB:  PubMed          Journal:  Infect Genet Evol        ISSN： 1567-1348            Impact factor:   3.342


  5 in total

1.  Use of fluorescent amplified fragment length polymorphism for molecular epidemiology of leptospirosis in India.

Authors:  P Vijayachari; Niyaz Ahmed; A P Sugunan; Sheikh Ghousunnissa; K Rajender Rao; Seyed E Hasnain; Subhash C Sehgal
Journal:  J Clin Microbiol       Date:  2004-08       Impact factor: 5.948

2.  Distinctiveness of Mycobacterium tuberculosis genotypes from human immunodeficiency virus type 1-seropositive and -seronegative patients in Lima, Peru.

Authors:  Niyaz Ahmed; Luz Caviedes; Mahfooz Alam; K Rajender Rao; Vartul Sangal; Patricia Sheen; Robert H Gilman; Seyed E Hasnain
Journal:  J Clin Microbiol       Date:  2003-04       Impact factor: 5.948

3.  Fine-structure molecular typing of Irish Helicobacter pylori isolates and their genetic relatedness to strains from four different continents.

Authors:  Ian M Carroll; Niyaz Ahmed; Sarah M Beesley; Aleem A Khan; Sheikh Ghousunnissa; Colm A O Moráin; Cyril J Smyth
Journal:  J Clin Microbiol       Date:  2003-12       Impact factor: 5.948

4.  Fluorescent amplified fragment length polymorphism (FAFLP) genotyping demonstrates the role of biofilm-producing methicillin-resistant periocular Staphylococcus epidermidis strains in postoperative endophthalmitis.

Authors:  Prashanth Kenchappa; Aparna Duggirala; Niyaz Ahmed; Avinash Pathengay; Taraprasad Das; Seyed E Hasnain; Savitri Sharma
Journal:  BMC Ophthalmol       Date:  2006-01-05       Impact factor: 2.209

5.  High-resolution genotyping of Pseudomonas aeruginosa strains linked to acute post cataract surgery endophthalmitis outbreaks in India.

Authors:  Prashanth Kenchappa; Virender S Sangwan; Niyaz Ahmed; K Rajender Rao; Avinash Pathengay; Annie Mathai; Tarannum Mansoori; Taraprasad Das; Seyed E Hasnain; Savitri Sharma
Journal:  Ann Clin Microbiol Antimicrob       Date:  2005-12-12       Impact factor: 3.944
  5 in total

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