Literature DB >> 1279521

Temperature-dependent template switching during in vitro cDNA synthesis by the AMV-reverse transcriptase.

M Ouhammouch1, E N Brody.   

Abstract

Reverse transcriptase template switching has been invoked to explain several aspects of retroviral replication and recombination, and has been reported in vitro for the Moloney murine leukemia virus (M-MuLV) reverse transcriptase. During in vitro cDNA synthesis, the avian myeloblastosis virus (AMV) reverse transcriptase can switch from one template to another in a homology-dependent and temperature-dependent manner. Chimeric cDNA molecules are generated within 30 min at high incubation temperatures, with an increasing efficiency from 42 degrees C to 50 degrees C. Such products are detectable only after much longer incubation times when primer extension reactions are carried out at lower temperatures (90 min at 37 degrees C).

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Year:  1992        PMID： 1279521      PMCID： PMC334354          DOI： 10.1093/nar/20.20.5443

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  14 in total

Review 1.  Sex and recombination in retroviruses.

Authors:  H M Temin
Journal:  Trends Genet       Date:  1991-03       Impact factor: 11.639

2.  Template switching by reverse transcriptase during DNA synthesis.

Authors:  G X Luo; J Taylor
Journal:  J Virol       Date:  1990-09       Impact factor: 5.103

3.  Nucleotide sequence and control of transcription of the bacteriophage T4 motA regulatory gene.

Authors:  M Uzan; E Brody; R Favre
Journal:  Mol Microbiol       Date:  1990-09       Impact factor: 3.501

4.  High-frequency deletion between homologous sequences during retrotransposition of Ty elements in Saccharomyces cerevisiae.

Authors:  H Xu; J D Boeke
Journal:  Proc Natl Acad Sci U S A       Date:  1987-12       Impact factor: 11.205

5.  Culture medium for enterobacteria.

Authors:  F C Neidhardt; P L Bloch; D F Smith
Journal:  J Bacteriol       Date:  1974-09       Impact factor: 3.490

6.  Transcriptional activation of bacteriophage T4 middle promoters by the motA protein.

Authors:  N Guild; M Gayle; R Sweeney; T Hollingsworth; T Modeer; L Gold
Journal:  J Mol Biol       Date:  1988-01-20       Impact factor: 5.469

7.  Analysis of gene control signals by DNA fusion and cloning in Escherichia coli.

Authors:  M J Casadaban; S N Cohen
Journal:  J Mol Biol       Date:  1980-04       Impact factor: 5.469

8.  Isolation and characterization of conditional lethal mutations in the mot gene of bacteriophage T4.

Authors:  T Mattson; G Van Houwe; R H Epstein
Journal:  J Mol Biol       Date:  1978-12-15       Impact factor: 5.469

9.  Intrinsic properties of reverse transcriptase in reverse transcription. Associated RNase H is essentially regarded as an endonuclease.

Authors:  F Oyama; R Kikuchi; R J Crouch; T Uchida
Journal:  J Biol Chem       Date:  1989-11-05       Impact factor: 5.157

10.  Transduction of a cellular oncogene: the genesis of Rous sarcoma virus.

Authors:  R Swanstrom; R C Parker; H E Varmus; J M Bishop
Journal:  Proc Natl Acad Sci U S A       Date:  1983-05       Impact factor: 11.205
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  16 in total

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Authors:  P D Yin; V K Pathak; A E Rowan; R J Teufel; W S Hu
Journal:  J Virol       Date:  1997-03       Impact factor: 5.103

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5.  The asiA gene product of bacteriophage T4 is required for middle mode RNA synthesis.

Authors:  M Ouhammouch; G Orsini; E N Brody
Journal:  J Bacteriol       Date:  1994-07       Impact factor: 3.490

6.  A minor fraction of basic fibroblast growth factor mRNA is deaminated in Xenopus stage VI and matured oocytes.

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Journal:  RNA       Date:  1999-01       Impact factor: 4.942

7.  Analysis of transcribed human endogenous retrovirus W env loci clarifies the origin of multiple sclerosis-associated retrovirus env sequences.

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Review 9.  The role of canonical and noncanonical pre-mRNA splicing in plant stress responses.

Authors:  A S Dubrovina; K V Kiselev; Yu N Zhuravlev
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10.  The venom gland transcriptome of the Desert Massasauga rattlesnake (Sistrurus catenatus edwardsii): towards an understanding of venom composition among advanced snakes (Superfamily Colubroidea).

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Journal:  BMC Mol Biol       Date:  2007-12-20       Impact factor: 2.946
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