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Literature DB >> 12794312

Nuclear immunostaining in rat neuronal cells using two anti-Kir2.2 ion channel polyclonal antibodies.

Anthony H Stonehouse¹, Blair D Grubb, J Howard Pringle, Robert I Norman, Peter R Stanfield, William J Brammar.

Abstract

The inwardly rectifying potassium ion channel Kir2.2 has recently been demonstrated to have nuclear and plasma membrane subcellular localization. Nuclear expression of Kir2.2 is controversial, as a functional role for Kir2.0 potassium channels in the nucleus has not been investigated. However, in this report we have demonstrated Kir2.2 nuclear localization in sections of rat hindbrain and dorsal root ganglia tissue, using two anti- Kir2.2 polyclonal antisera with different epitope specificities. These data confirm nuclear localization and are suggestive of new functions of Kir2.0 potassium ion channels in the nucleus.

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Year: 2003 PMID： 12794312 DOI： 10.1385/JMN:20:2:189

Source DB: PubMed Journal: J Mol Neurosci ISSN： 0895-8696 Impact factor: 3.444

13 in total

1. Modelling action potentials and membrane currents of mammalian skeletal muscle fibres in coherence with potassium concentration changes in the T-tubular system.

Authors: W Wallinga; S L Meijer; M J Alberink; M Vliek; E D Wienk; D L Ypey
Journal: Eur Biophys J Date: 1999 Impact factor: 1.733

2. Characterisation of Kir2.0 proteins in the rat cerebellum and hippocampus by polyclonal antibodies.

Authors: A H Stonehouse; J H Pringle; R I Norman; P R Stanfield; E C Conley; W J Brammar
Journal: Histochem Cell Biol Date: 1999-12 Impact factor: 4.304

Review 3. Nuclear calcium signalling.

Authors: M D Bootman; D Thomas; S C Tovey; M J Berridge; P Lipp
Journal: Cell Mol Life Sci Date: 2000-03 Impact factor: 9.261

Review 4. Calcium transport pathways in the nucleus.

Authors: O V Gerasimenko; J V Gerasimenko; A V Tepikin; O H Petersen
Journal: Pflugers Arch Date: 1996-05 Impact factor: 3.657

5. Large-conductance cation channels in the envelope of nuclei from rat cerebral cortex.

Authors: A Draguhn; G Börner; R Beckmann; K Buchner; U Heinemann; F Hucho
Journal: J Membr Biol Date: 1997-07-15 Impact factor: 1.843

6. IP(3) receptors, IP(3) transients, and nucleus-associated Ca(2+) signals in cultured skeletal muscle.

Authors: E Jaimovich; R Reyes; J L Liberona; J A Powell
Journal: Am J Physiol Cell Physiol Date: 2000-05 Impact factor: 4.249

7. ATP-dependent accumulation and inositol trisphosphate- or cyclic ADP-ribose-mediated release of Ca2+ from the nuclear envelope.

Authors: O V Gerasimenko; J V Gerasimenko; A V Tepikin; O H Petersen
Journal: Cell Date: 1995-02-10 Impact factor: 41.582

Nuclear immunostaining in rat neuronal cells using two anti-Kir2.2 ion channel polyclonal antibodies.

1. Modelling action potentials and membrane currents of mammalian skeletal muscle fibres in coherence with potassium concentration changes in the T-tubular system.

2. Characterisation of Kir2.0 proteins in the rat cerebellum and hippocampus by polyclonal antibodies.

Review 3. Nuclear calcium signalling.

Review 4. Calcium transport pathways in the nucleus.

5. Large-conductance cation channels in the envelope of nuclei from rat cerebral cortex.

6. IP(3) receptors, IP(3) transients, and nucleus-associated Ca(2+) signals in cultured skeletal muscle.

7. ATP-dependent accumulation and inositol trisphosphate- or cyclic ADP-ribose-mediated release of Ca2+ from the nuclear envelope.

8. Ca(2+)-activated K(+)-channels in the nuclear envelope isolated from single pancreatic acinar cells.

9. Tetrameric subunit structure of the native brain inwardly rectifying potassium channel Kir 2.2.

10. Immunochemical demonstration that amino acids 360-377 of the acetylcholine receptor gamma-subunit are cytoplasmic.

1. Nuclear localization and functional characteristics of voltage-gated potassium channel Kv1.3.

2. Mislocalization of Kir channels in malignant glia.