Literature DB >> 1279182

Crystallization and preliminary X-ray crystallographic analysis of lipase from Pseudomonas cepacia.

K K Kim1, K Y Hwang, H S Jeon, S Kim, R M Sweet, C H Yang, S W Suh.   

Abstract

Large crystals of lipase from Pseudomonas cepacia have been grown at room temperature from solutions containing 2-methyl-2,4-pentanediol and sodium citrate. They grow within two weeks to typical dimensions of 1.0 mm x 0.5 mm x 0.3 mm. The crystals belong to the monoclinic space group P2(1), with unit cell parameters a = 84.91 A, b = 47.33 A, c = 86.00 A, and beta = 116.09 degrees. And they diffract to about 1.6 A upon exposure to synchroton X-rays. X-ray data have been collected to 2.2 A Bragg spacing from a native crystal.

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Year:  1992        PMID: 1279182     DOI: 10.1016/0022-2836(92)90537-t

Source DB:  PubMed          Journal:  J Mol Biol        ISSN: 0022-2836            Impact factor:   5.469


  1 in total

1.  The effect of ultrasonic pre-treatment on the catalytic activity of lipases in aqueous and non-aqueous media.

Authors:  Shweta Shah; Munishwar Nath Gupta
Journal:  Chem Cent J       Date:  2008-01-30       Impact factor: 4.215

  1 in total

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