PURPOSE: Retinal degeneration induced by sodium iodate (NaIO( 3)) in mice was evaluated morphologically. METHODS: Male and female ICR and C57BL mice were intraperitoneally administered 100 mg/kg NaIO(3) at 7 weeks of age, and were killed 6, 12, 24 hrs, and 3, 7 and 28 days after the treatment. Retinas were examined histologically, ultrastructurally, immunohistochemically, and by the TUNEL method. RESULTS: Retinal degeneration was evoked in all NaIO(3)-treated mice. The primary site of damage appeared in the retinal pigment epithelial (RPE) cells followed by photoreceptor cell degeneration. Initially, the RPE cells showed necrosis starting 6 hrs post-NaIO(3), followed by photoreceptor outer segment disruption and photoreceptor cell apoptosis at 24 hrs; photoreceptor cell apoptosis peaked at day 3 and was completed by day 7. At day 3, Müller cell proliferation, macrophage migration within the retina, and regeneration of damaged RPE cells occurred. Finally at day 7 and day 28, the retina showed a mosaic pattern of relatively normal retina and areas lacking RPE cells and photoreceptor cells. CONCLUSIONS: RPE cell necrosis followed by photoreceptor cell apoptosis and the resulting mosaic pattern of the retina phenotypically resembles gyrate atrophy of the choroid and retina.
PURPOSE:Retinal degeneration induced by sodium iodate (NaIO( 3)) in mice was evaluated morphologically. METHODS: Male and female ICR and C57BL mice were intraperitoneally administered 100 mg/kg NaIO(3) at 7 weeks of age, and were killed 6, 12, 24 hrs, and 3, 7 and 28 days after the treatment. Retinas were examined histologically, ultrastructurally, immunohistochemically, and by the TUNEL method. RESULTS:Retinal degeneration was evoked in all NaIO(3)-treated mice. The primary site of damage appeared in the retinal pigment epithelial (RPE) cells followed by photoreceptor cell degeneration. Initially, the RPE cells showed necrosis starting 6 hrs post-NaIO(3), followed by photoreceptor outer segment disruption and photoreceptor cell apoptosis at 24 hrs; photoreceptor cell apoptosis peaked at day 3 and was completed by day 7. At day 3, Müller cell proliferation, macrophage migration within the retina, and regeneration of damaged RPE cells occurred. Finally at day 7 and day 28, the retina showed a mosaic pattern of relatively normal retina and areas lacking RPE cells and photoreceptor cells. CONCLUSIONS: RPE cell necrosis followed by photoreceptor cell apoptosis and the resulting mosaic pattern of the retina phenotypically resembles gyrateatrophy of the choroid and retina.
Authors: Jeffrey R Harris; Gary A J Brown; Marda Jorgensen; Shalesh Kaushal; E Ann Ellis; Maria B Grant; Edward W Scott Journal: Invest Ophthalmol Vis Sci Date: 2006-05 Impact factor: 4.799
Authors: Anna Machalińska; Wojciech Lubiński; Patrycja Kłos; Miłosz Kawa; Bartłomiej Baumert; Krzysztof Penkala; Ryszard Grzegrzółka; Danuta Karczewicz; Barbara Wiszniewska; Bogusław Machaliński Journal: Neurochem Res Date: 2010-08-20 Impact factor: 3.996