Literature DB >> 12789503

Transformation of triploid bermudagrass (Cynodon dactylon x C. transvaalensis cv. TifEagle) by means of biolistic bombardment.

G Zhang1, S Lu, T A Chen, C R Funk, W A Meyer.   

Abstract

A transformation system for triploid bermudagrass ( Cynodon dactylon x C. transvaalensis cv. TifEagle) was established with a biolistic bombardment delivery system. Embryogenic callus was induced from stolons and maintained on Murashige and Skoog's medium supplemented with 30 microM dicamba, 20 microM benzylaminopurine, and 100 mg/l myo-inositol. Using the hygromycin phosphotransferase ( hpt) gene as the selectable marker gene, we obtained 75 transgenic lines from 18 petri dishes bombarded. Integration of the hpt gene into genomic DNA and transcription of hpt was confirmed by Southern and Northern blot analyses, respectively. Through suspension culture screening, we obtained homogeneously transformed plants showing stable transcription of the hpt gene.

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Year:  2003        PMID： 12789503     DOI： 10.1007/s00299-003-0585-x

Source DB:  PubMed          Journal:  Plant Cell Rep        ISSN： 0721-7714            Impact factor:   4.570


  6 in total

1.  The 3'-terminal region of the hygromycin-B-resistance gene is important for its activity in Escherichia coli and Nicotiana tabacum.

Authors:  R Bilang; S Iida; A Peterhans; I Potrykus; J Paszkowski
Journal:  Gene       Date:  1991-04       Impact factor: 3.688

2.  Osmotic treatment enhances particle bombardment-mediated transient and stable transformation of maize.

Authors:  P Vain; M D McMullen; J J Finer
Journal:  Plant Cell Rep       Date:  1993-01       Impact factor: 4.570

3.  Retrotransposons of rice involved in mutations induced by tissue culture.

Authors:  H Hirochika; K Sugimoto; Y Otsuki; H Tsugawa; M Kanda
Journal:  Proc Natl Acad Sci U S A       Date:  1996-07-23       Impact factor: 11.205

4.  Construction of expression vectors based on the rice actin 1 (Act1) 5' region for use in monocot transformation.

Authors:  D McElroy; A D Blowers; B Jenes; R Wu
Journal:  Mol Gen Genet       Date:  1991-12

5.  Maize polyubiquitin genes: structure, thermal perturbation of expression and transcript splicing, and promoter activity following transfer to protoplasts by electroporation.

Authors:  A H Christensen; R A Sharrock; P H Quail
Journal:  Plant Mol Biol       Date:  1992-02       Impact factor: 4.076

6.  Transgenic plants of tall fescue (Festuca arundinacea Schreb.) obtained by direct gene transfer to protoplasts.

Authors:  Z Y Wang; T Takamizo; V A Iglesias; M Osusky; J Nagel; I Potrykus; G Spangenberg
Journal:  Biotechnology (N Y)       Date:  1992-06
  6 in total
  8 in total

1.  In vitro selection of salinity tolerant variants from triploid bermudagrass (Cynodon transvaalensis x C. dactylon) and their physiological responses to salt and drought stress.

Authors:  Shaoyun Lu; Xinxiang Peng; Zhenfei Guo; Gengyun Zhang; Zhongcheng Wang; Congying Wang; Chaoshu Pang; Zhen Fan; Jihua Wang
Journal:  Plant Cell Rep       Date:  2007-03-22       Impact factor: 4.570

2.  Generation of large numbers of transgenic Kentucky bluegrass (Poa pratensis L.) plants following biolistic gene transfer.

Authors:  Caixia Gao; Li Jiang; Marianne Folling; Liebao Han; Klaus Kristian Nielsen
Journal:  Plant Cell Rep       Date:  2005-11-19       Impact factor: 4.570

Review 3.  Tissue Culture and Somatic Embryogenesis in Warm-Season Grasses-Current Status and Its Applications: A Review.

Authors:  Melody Ballitoc Muguerza; Takahiro Gondo; Genki Ishigaki; Yasuyo Shimamoto; Nafiatul Umami; Pattama Nitthaisong; Mohammad Mijanur Rahman; Ryo Akashi
Journal:  Plants (Basel)       Date:  2022-05-07

4.  Comparative analysis of transgenic tall fescue (Festuca arundinacea Schreb.) plants obtained by Agrobacterium-mediated transformation and particle bombardment.

Authors:  Caixia Gao; Danfeng Long; Ingo Lenk; Klaus Kristian Nielsen
Journal:  Plant Cell Rep       Date:  2008-07-22       Impact factor: 4.570

5.  Ploidy variation among herbicide-resistant bermudagrass plants of cv. TifEagle transformed with the bar gene.

Authors:  J J Goldman; W W Hanna; G H Fleming; P Ozias-Akins
Journal:  Plant Cell Rep       Date:  2003-11-14       Impact factor: 4.570

6.  Development of highly regenerable callus lines and biolistic transformation of turf-type common bermudagrass [Cynodon dactylon (L.) Pers.].

Authors:  L Li; R Qu
Journal:  Plant Cell Rep       Date:  2003-10-01       Impact factor: 4.570

7.  A rapid, highly efficient and economical method of Agrobacterium-mediated in planta transient transformation in living onion epidermis.

Authors:  Kedong Xu; Xiaohui Huang; Manman Wu; Yan Wang; Yunxia Chang; Kun Liu; Ju Zhang; Yi Zhang; Fuli Zhang; Liming Yi; Tingting Li; Ruiyue Wang; Guangxuan Tan; Chengwei Li
Journal:  PLoS One       Date:  2014-01-08       Impact factor: 3.240

8.  Histone acetylation accompanied with promoter sequences displaying differential expression profiles of B-class MADS-box genes for phalaenopsis floral morphogenesis.

Authors:  Chia-Chi Hsu; Pei-Shan Wu; Tien-Chih Chen; Chun-Wei Yu; Wen-Chieh Tsai; Keqiang Wu; Wen-Luan Wu; Wen-Huei Chen; Hong-Hwa Chen
Journal:  PLoS One       Date:  2014-12-11       Impact factor: 3.240
  8 in total

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