Literature DB >> 12788777

Phylogeny and characterization of three nifH-homologous genes from Paenibacillus azotofixans.

Quok-Cheong Choo1, Mohd-Razip Samian, Nazalan Najimudin.   

Abstract

In this paper, we report the cloning and characterization of three Paenibacillus azotofixans DNA regions containing genes involved in nitrogen fixation. Sequencing analysis revealed the presence of nifB1H1D1K1 gene organization in the 4,607-bp SacI DNA fragment. This is the first report of linkage of a nifB open reading frame upstream of the structural nif genes. The second (nifB2H2) and third (nifH3) nif homologues are confined within the 6,350-bp HindIII and 2,840-bp EcoRI DNA fragments, respectively. Phylogenetic analysis demonstrated that NifH1 and NifH2 form a monophyletic group among cyanobacterial NifH proteins. NifH3, on the other hand, clusters among NifH proteins of the highly divergent methanogenic archaea.

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Year:  2003        PMID： 12788777      PMCID： PMC161525          DOI： 10.1128/AEM.69.6.3658-3662.2003

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  27 in total

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