Literature DB >> 1278331

Holding plastic-embedded specimens for sectioning in a rotary microtome.

M B Cole.   

Abstract

Three methods are evaluated for holding capsules of plastic-embedded tissue for rotary microtomy. Use of a V-block is rapid but deforms the capsule. Gluing the capsule to a supporting block is useful for reorientation of the capsule but otherwise time consuming. adapter is easy to use, does not deform the tissue, and is the preferred method for routine microtomy.

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Year:  1976        PMID: 1278331     DOI: 10.1007/BF01990229

Source DB:  PubMed          Journal:  Experientia        ISSN: 0014-4754


  10 in total

1.  A PLASTIC EMBEDDING MEDIUM FOR THIN SECTIONING IN LIGHT MICROSCOPY.

Authors:  W J CATHEY
Journal:  Stain Technol       Date:  1963-07

2.  Plastic embedding, orientation in the mold and tape-supported sectioning of sclerotized insects and other hard objects.

Authors:  W E BECKEL; J E HABOWSKY
Journal:  Stain Technol       Date:  1960-11

3.  SERIAL SECTIONS FROM PLASTIC-EMBEDDED SPECIMENS; ARTHROPODS IN METHACRYLATE.

Authors:  C J ROHDE
Journal:  Stain Technol       Date:  1965-01

4.  A modified polyester embedding medium for sectioning.

Authors:  G D KUHN; E L LUTZ
Journal:  Stain Technol       Date:  1958-01

5.  A simple method of preparing 1-2 microm sections of large tissue blocks using glycol methacrylate.

Authors:  B Sims
Journal:  J Microsc       Date:  1974-07       Impact factor: 1.758

6.  Embedding media for 1-2 micron sectioning. 3. Hydroxyethyl methacrylate-benzoyl peroxide activated with pyridine.

Authors:  C L Ruddell
Journal:  Stain Technol       Date:  1971-03

7.  Hydroxyethyl methacrylate combined with polyethylene glycol 400 and water; and embedding medium for routine 1-2 micron sectioning.

Authors:  C L Ruddell
Journal:  Stain Technol       Date:  1967-05

8.  Glycol methacrylate in histopathology. A study of central necrosis of the liver using a water-miscible plastic as embedding medium.

Authors:  C A Ashley; N Feder
Journal:  Arch Pathol       Date:  1966-05

9.  Glycol methacrylate in light microscopy: a routine method for embedding and sectioning animal tissues.

Authors:  M B Cole; S M Sykes
Journal:  Stain Technol       Date:  1974-11

10.  Embedding media for 1-2 micron sectioning. 2. Hydroxyethyl methacrylate combined with 2-butoxyethanol.

Authors:  C L Ruddell
Journal:  Stain Technol       Date:  1967-09
  10 in total

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