OBJECTIVE: To describe the methods which were used to develop collagen-based materials for wound dressing. METHODS: Fresh frozen bovine tendon was treated with 0.05 mol/L acetic acid at pH 3.2 for 48-72 hours, homogenized, filtered, mixed with 8% chondroitin sulphate, for creating a deaerated 1.5%-2.5% collagen solution. The solution was lyophilized in either a pre-frozen or non-pre-frozen mould. The collagen sponge was then cross-linked with 0.25% glutaraldehyde for 24 hours. Three other types of wound dressings were developed using a similar method: collagen membrane with a polyurethane membrane onlay, polyurethane-coated collagen membrane and collagen membrane on gauze. RESULTS: It was demonstrated that the use of frozen bovine tendon was stable, and that the prepared collagen sponge contained pores of 50-400 microm in diameter. CONCLUSIONS: Collagen could be used as wound dressing.
OBJECTIVE: To describe the methods which were used to develop collagen-based materials for wound dressing. METHODS: Fresh frozen bovine tendon was treated with 0.05 mol/L acetic acid at pH 3.2 for 48-72 hours, homogenized, filtered, mixed with 8% chondroitin sulphate, for creating a deaerated 1.5%-2.5% collagen solution. The solution was lyophilized in either a pre-frozen or non-pre-frozen mould. The collagen sponge was then cross-linked with 0.25% glutaraldehyde for 24 hours. Three other types of wound dressings were developed using a similar method: collagen membrane with a polyurethane membrane onlay, polyurethane-coated collagen membrane and collagen membrane on gauze. RESULTS: It was demonstrated that the use of frozen bovine tendon was stable, and that the prepared collagen sponge contained pores of 50-400 microm in diameter. CONCLUSIONS: Collagen could be used as wound dressing.
Authors: J F Mano; G A Silva; H S Azevedo; P B Malafaya; R A Sousa; S S Silva; L F Boesel; J M Oliveira; T C Santos; A P Marques; N M Neves; R L Reis Journal: J R Soc Interface Date: 2007-12-22 Impact factor: 4.118