| Literature DB >> 12777774 |
Ioulia M Vassilieva1, Alexey D Nikulin, Udo Blasi, Isabella Moll, Maria B Garber.
Abstract
Hfq protein from Escherichia coli (EcoHfq) has been overproduced in E. coli, purified to homogeneity and crystallized using the hanging-drop vapour-diffusion technique. Crystallization conditions for EcoHfq were found which yielded X-ray quality crystals. Crystals of EcoHfq and of Cd-, Hg- and Se-containing derivatives grew in two months, with unit-cell parameters a = b = 127.41, c = 170.36 A. The crystals belong to space group I4 and diffract to 2.1 A resolution. Two hexamers are predicted per asymmetric unit.Entities:
Mesh：
Substances：
Year: 2003 PMID： 12777774 DOI： 10.1107/s0907444903006929
Source DB: PubMed Journal: Acta Crystallogr D Biol Crystallogr ISSN： 0907-4449