Y F Huang1, Y M Zhang, A He, X H Cao. 1. Third Affiliated Hospital, Sun Yat-sen University of Medical Sciences, Guangzhou 510630, Guangdong, China.
Abstract
OBJECTIVE: To develop a method to determine the content of puerarin in Xinmaitong oral liquid. METHODS: HPLC method was used. The separation was performed on Suntek kromasil C18 column with methanol-water containing 0.5% acetic acid (25:75) as a mobile phase and the wavelength of UV detector was 250 nm. RESULTS: The linearity of puerarin was good and average recovery was 97.8%. CONCLUSION: The method is simple, reliable and sensitive. It also shows good resolation. It can be used in quality control of Xinmaitong oral liquid.
OBJECTIVE: To develop a method to determine the content of puerarin in Xinmaitong oral liquid. METHODS: HPLC method was used. The separation was performed on Suntek kromasil C18 column with methanol-water containing 0.5% acetic acid (25:75) as a mobile phase and the wavelength of UV detector was 250 nm. RESULTS: The linearity of puerarin was good and average recovery was 97.8%. CONCLUSION: The method is simple, reliable and sensitive. It also shows good resolation. It can be used in quality control of Xinmaitong oral liquid.