Literature DB >> 1277557

Simultaneous estimation of urinary steroids by semi-automated gas chromatography. Investigation of neo-natal infants and children with abnormal steroid synthesis.

C H Shackleton, J W Honour.   

Abstract

Development of a method for multicomponent analysis of urinary steroids on open-tubular columns is described. The urinary steroid conjugates were hydrolysed enzymatically, extracted on Amberlite XAD-2 columns, purified on columns of Sephadex LH-20, then analysed as methyloxime trimethylsilyl ethers on a gas chromatograph on which up to 24 samples may be automatically injected. All major urinary steroids from 17-oxosteroids to cortisol metabolites were analysed and these were quantified relative to internal standards, 5alpha-androstane-3alpha, 17alpha-diol and cholesteryl butyrate added to the samples prior to derivatisation. The precision of the full technique and reproducibility of repetitive automatic solid injection was found to be acceptable for the purpose of profile analysis. Examples of urinary steroid profiles in normal infants and infants with disorders of adrenal steroid production and excretion are given.

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Year:  1976        PMID： 1277557     DOI： 10.1016/0009-8981(76)90505-2

Source DB:  PubMed          Journal:  Clin Chim Acta        ISSN： 0009-8981            Impact factor:   3.786


  17 in total

1.  Urinary excretion of 17-hydroxypregnanolones in patients with different forms of congenital adrenal hyperplasia due to steroid 21-hydroxylase deficiency.

Authors:  J Homoki; J Sólyom; U Wachter; W M Teller
Journal:  Eur J Pediatr       Date:  1992-01       Impact factor: 3.183

2.  Review of CAH screening programmes and the Scottish experience.

Authors:  A M Wallace
Journal:  J Inherit Metab Dis       Date:  1986       Impact factor: 4.982

3.  [Urinary steroid profile in Cushing syndrome and in tumors of the adrenal cortex].

Authors:  J Homoki; R Holl; W M Teller
Journal:  Klin Wochenschr       Date:  1987-08-03

4.  Cyp7b, a novel brain cytochrome P450, catalyzes the synthesis of neurosteroids 7alpha-hydroxy dehydroepiandrosterone and 7alpha-hydroxy pregnenolone.

Authors:  K A Rose; G Stapleton; K Dott; M P Kieny; R Best; M Schwarz; D W Russell; I Björkhem; J Seckl; R Lathe
Journal:  Proc Natl Acad Sci U S A       Date:  1997-05-13       Impact factor: 11.205

5.  Transient pseudo-hypoaldosteronism following resection of the ileum: normal level of lymphocytic aldosterone receptors outside the acute phase.

Authors:  M C Vantyghem; C Hober; A Evrard; A Ghulam; D Lescut; A Racadot; J P Triboulet; D Armanini; J Lefebvre
Journal:  J Endocrinol Invest       Date:  1999-02       Impact factor: 4.256

6.  Stimulation of gonadal steroid synthesis by chronic excess of adrenocorticotropin in patients with adrenocortical insufficiency.

Authors:  H Vierhapper; P Nowotny; W Waldhäusl
Journal:  J Clin Invest       Date:  1986-04       Impact factor: 14.808

7.  Detection of late onset steroid 21-hydroxylase deficiency by capillary gas chromatographic profiling of urinary steroids in children and adolescents.

Authors:  J Homoki; J Solyom; W M Teller
Journal:  Eur J Pediatr       Date:  1988-04       Impact factor: 3.183

8.  Urinary cortisol metabolites in the assessment of peripheral thyroid hormone action: overt and subclinical hypothyroidism.

Authors:  M C Vantyghem; A Ghulam; C Hober; C Schoonberg; M D'Herbomez; A Racadot; A Boersma; J Lefebvre
Journal:  J Endocrinol Invest       Date:  1998-04       Impact factor: 4.256

9.  Steroid biochemistry of virilising adrenal tumours in childhood.

Authors:  J W Honour; D A Price; N F Taylor; H B Marsden; D B Grant
Journal:  Eur J Pediatr       Date:  1984-08       Impact factor: 3.183

10.  Effect of regular training on the anthropometric parameters and urine steroids in childhood.

Authors:  Z Juricskay; B Mezey
Journal:  Eur J Appl Physiol Occup Physiol       Date:  1994
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