Literature DB >> 12773402

Human oocytes reversibly arrested in prophase I by phosphodiesterase type 3 inhibitor in vitro.

Daniela Nogueira1, Carola Albano, Tom Adriaenssens, Rita Cortvrindt, Claire Bourgain, Paul Devroey, Johan Smitz.   

Abstract

This study addresses the role of cAMP hydrolytic isoenzyme phosphodiesterase type 3 (PDE 3) modulation on human oocyte maturation in vitro. Presence of phosphodiesterase type 3 A (PDE 3A) mRNA was confirmed in human germinal vesicle-stage (GV) oocytes. Making use of a selective PDE 3 inhibitor, Org 9935 (10 microM), oocytes retrieved from immature follicles were arrested in prophase I with a high efficiency for up to 72 h. Cumulus oocyte complexes (COCs) were retrieved in the follicular phase of the cycle before or after exposure to endogenous LH or hCG administration in vivo and randomly distributed into maturation medium with or without the PDE 3 inhibitor. Previous exposure of small follicles to LH activity in vivo had no influence on the arresting capacity of the PDE 3 inhibitor. Reversal from pharmacological arrest leads to a progression through meiosis in a normal time frame with formation of a well-aligned metaphase plate. Ultrastructure analysis of COC derived from follicles between 8 and 12 mm showed that the induced extension of prophase I arrest in vitro resulted in cytoplasm changes but not in apparent nuclear changes during culture.

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Year:  2003        PMID: 12773402     DOI: 10.1095/biolreprod.103.015982

Source DB:  PubMed          Journal:  Biol Reprod        ISSN: 0006-3363            Impact factor:   4.285


  23 in total

1.  Phosphodiesterase 3 (PDE3) inhibition with cilostazol does not block in vivo oocyte maturation in rhesus macaques (Macaca mulatta).

Authors:  Carol B Hanna; Shan Yao; Cathy M Ramsey; Jon D Hennebold; Mary B Zelinski; Jeffrey T Jensen
Journal:  Contraception       Date:  2015-01-30       Impact factor: 3.375

Review 2.  The mammalian ovary from genesis to revelation.

Authors:  Mark A Edson; Ankur K Nagaraja; Martin M Matzuk
Journal:  Endocr Rev       Date:  2009-09-23       Impact factor: 19.871

3.  The effect of temporary meiotic attenuation on the in vitro maturation outcome of bovine oocytes.

Authors:  T Farghaly; E Khalifa; S Mostafa; M Hussein; M Bedaiwy; A Ahmady
Journal:  In Vitro Cell Dev Biol Anim       Date:  2015-03-18       Impact factor: 2.416

Review 4.  Regulation of the G2/M transition in rodent oocytes.

Authors:  Stephen M Downs
Journal:  Mol Reprod Dev       Date:  2010-07       Impact factor: 2.609

5.  Identification of phosphodiesterase 9A as a cyclic guanosine monophosphate-specific phosphodiesterase in germinal vesicle oocytes: a proposed role in the resumption of meiosis.

Authors:  Carol B Hanna; Shan Yao; Xuemei Wu; Jeffrey T Jensen
Journal:  Fertil Steril       Date:  2012-06-15       Impact factor: 7.329

6.  Multiplexed detection and the establishment of a novel high-throughput method for human germ cell quality screening based on aggregation-induced emission.

Authors:  Zixuan Zeng; Xiaojiao Ren; Tailang Yin; Xiang Gao; Mengting Tsai; Yi Zhang; Meijia Gu
Journal:  Am J Transl Res       Date:  2019-11-15       Impact factor: 4.060

7.  Transporting cumulus complexes using novel meiotic arresting conditions permits maintenance of oocyte developmental competence.

Authors:  Nicolas W Santiquet; Jason R Herrick; Angelica Giraldo; Jennifer P Barfield; William B Schoolcraft; Rebecca L Krisher
Journal:  J Assist Reprod Genet       Date:  2017-06-01       Impact factor: 3.412

8.  The phosphodiesterase 3 inhibitor ORG 9935 inhibits oocyte maturation in the naturally selected dominant follicle in rhesus macaques.

Authors:  Jeffrey T Jensen; Mary B Zelinski; Jessica E Stanley; John W Fanton; Richard L Stouffer
Journal:  Contraception       Date:  2008-03-04       Impact factor: 3.375

9.  Meiotic arrest in human oocytes is maintained by a Gs signaling pathway.

Authors:  Andrea DiLuigi; Vanessa N Weitzman; Margaret C Pace; Linda J Siano; Donald Maier; Lisa M Mehlmann
Journal:  Biol Reprod       Date:  2008-01-09       Impact factor: 4.285

10.  Evaluation of the phosphodiesterase 3 inhibitor ORG 9935 as a contraceptive in female macaques: initial trials.

Authors:  Jeffrey T Jensen; Richard L Stouffer; Jessica E Stanley; Mary B Zelinski
Journal:  Contraception       Date:  2009-11-06       Impact factor: 3.375

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