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Literature DB >> 12768037

Trans activity of the norovirus Camberwell proteinase and cleavage of the N-terminal protein encoded by ORF1.

Ee Ling Seah¹, John A Marshall, Peter J Wright.

Abstract

The virus-encoded proteinase of Camberwell virus, a genogroup 2 norovirus, was synthesized in Escherichia coli. The purified proteinase had correct N and C termini and showed trans activity in cell-free assays. trans activity was also demonstrated in COS cells transfected with constructs encoding either the proteinase or a proteinase-polymerase fusion. The N-terminal protein of ORF1 was cleaved in COS cells, possibly at the site E(194)/S.

Entities: Species

Mesh：

Substances：
Endopeptidases

Year: 2003 PMID： 12768037 PMCID： PMC156193 DOI： 10.1128/jvi.77.12.7150-7155.2003

Source DB: PubMed Journal: J Virol ISSN： 0022-538X Impact factor: 5.103

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