| Literature DB >> 12766765 |
Samuel J McConkey1, William H H Reece, Vasee S Moorthy, Daniel Webster, Susanna Dunachie, Geoff Butcher, Jenni M Vuola, Tom J Blanchard, Philip Gothard, Kate Watkins, Carolyn M Hannan, Simone Everaere, Karen Brown, Kent E Kester, James Cummings, Jackie Williams, D Gray Heppner, Ansar Pathan, Katie Flanagan, Nirmalan Arulanantham, Mark T M Roberts, Michael Roy, Geoffrey L Smith, Joerg Schneider, Tim Peto, Robert E Sinden, Sarah C Gilbert, Adrian V S Hill.
Abstract
In animals, effective immune responses against malignancies and against several infectious pathogens, including malaria, are mediated by T cells. Here we show that a heterologous prime-boost vaccination regime of DNA either intramuscularly or epidermally, followed by intradermal recombinant modified vaccinia virus Ankara (MVA), induces high frequencies of interferon (IFN)-gamma-secreting, antigen-specific T-cell responses in humans to a pre-erythrocytic malaria antigen, thrombospondin-related adhesion protein (TRAP). These responses are five- to tenfold higher than the T-cell responses induced by the DNA vaccine or recombinant MVA vaccine alone, and produce partial protection manifest as delayed parasitemia after sporozoite challenge with a different strain of Plasmodium falciparum. Such heterologous prime-boost immunization approaches may provide a basis for preventative and therapeutic vaccination in humans.Entities:
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Year: 2003 PMID： 12766765 DOI： 10.1038/nm881
Source DB: PubMed Journal: Nat Med ISSN： 1078-8956 Impact factor: 53.440