BACKGROUND: Anti-mitochondrial antibody is the diagnostic hallmark of primary biliary cirrhosis. Its role in the aetiology of primary biliary cirrhosis is controversial. METHODS: Two cases of neonatal hepatitis seropositive for anti-mitochondrial antibody are described. Anti-mitochondrial antibody Ig isotype and epitopic specificity were investigated by immunofluorescence and enzyme immunoassays. RESULTS: In both infants anti-mitochondrial antibody was of the G class, mainly G1 and G3 subclasses, and reacted with two synthetic peptides reproducing major M2 epitopicregions: innerlipoyl domain pyruvate dehydrogenase complex (PDC)-E2(162-176) and PDC-E3 binding protein (PDC-E3BP)86-100. One infant also reacted with outer lipoyl domain PDC-E2(35-49), and 2-oxoglutarate dehydrogenase complex (OGDC)-E2(99-113). An identical pattern of reactivity was present in their mothers, indicating the maternal origin of the antibodies. Anti-mitochondrial antibody disappeared in the infants with the disappearance of the liver pathology. CONCLUSIONS: The simultaneous disappearance of hepatitis and anti-mitochondrial antibody in the infants suggests a possible causal link between the two.
BACKGROUND: Anti-mitochondrial antibody is the diagnostic hallmark of primary biliary cirrhosis. Its role in the aetiology of primary biliary cirrhosis is controversial. METHODS: Two cases of neonatal hepatitis seropositive for anti-mitochondrial antibody are described. Anti-mitochondrial antibody Ig isotype and epitopic specificity were investigated by immunofluorescence and enzyme immunoassays. RESULTS: In both infants anti-mitochondrial antibody was of the G class, mainly G1 and G3 subclasses, and reacted with two synthetic peptides reproducing major M2 epitopicregions: innerlipoyl domain pyruvate dehydrogenase complex (PDC)-E2(162-176) and PDC-E3 binding protein (PDC-E3BP)86-100. One infant also reacted with outer lipoyl domain PDC-E2(35-49), and 2-oxoglutarate dehydrogenase complex (OGDC)-E2(99-113). An identical pattern of reactivity was present in their mothers, indicating the maternal origin of the antibodies. Anti-mitochondrial antibody disappeared in the infants with the disappearance of the liver pathology. CONCLUSIONS: The simultaneous disappearance of hepatitis and anti-mitochondrial antibody in the infants suggests a possible causal link between the two.
Authors: Daniel Smyk; Eirini I Rigopoulou; Yoh Zen; Robin Daniel Abeles; Charalambos Billinis; Albert Pares; Dimitrios P Bogdanos Journal: World J Gastroenterol Date: 2012-09-21 Impact factor: 5.742
Authors: Daniel S Smyk; Eirini I Rigopoulou; Ana Lleo; Robin D Abeles; Athanasios Mavropoulos; Charalambos Billinis; Pietro Invernizzi; Dimitrios P Bogdanos Journal: Immun Ageing Date: 2011-12-02 Impact factor: 6.400
Authors: Eirini I Rigopoulou; Daniel S Smyk; Claire E Matthews; Charalambos Billinis; Andrew K Burroughs; Marco Lenzi; Dimitrios P Bogdanos Journal: Adv Virol Date: 2012-05-28
Authors: Daniel Smyk; Tassos Grammatikopoulos; Alexandros Daponte; Eirini I Rigopoulou; Dimitrios P Bogdanos Journal: Auto Immun Highlights Date: 2011-03-23
Authors: Daniel S Smyk; Maria G Mytilinaiou; Tassos Grammatikopoulos; A S Knisely; Giorgina Mieli-Vergani; Dimitrios P Bogdanos; Diego Vergani Journal: Clin Dev Immunol Date: 2013-09-19
Authors: Daniel S Smyk; Dimitrios P Bogdanos; Albert Pares; Christos Liaskos; Charalambos Billinis; Andrew K Burroughs; Eirini I Rigopoulou Journal: Tuberc Res Treat Date: 2012-10-30