BACKGROUND: The aim of this study was to estimate the role of acetate-induced metabolic changes in brain mechanisms of resistance to the narcotic effect of ethanol. MATERIAL/ METHODS: Wistar rats were treated daily with ethanol (3.5 g/kg i.p. for 7 days). During alcohol treatment, the duration of ethanol-induced sleep was decreased. Levels of acetate, acetyl-CoA, adenosine, and the pool of AMP+ADP, and the activity of acetyl-CoA synthetase and 5'-nucleotidase in the brain were measured. Synaptosomal adenosine and acetylcholine release were measured in the presence of acetate, adenosine and 2-chloroadenosine. RESULTS: The concentration of acetate was higher in all investigated brain regions of ethanol rats in comparison to controls. The activity of acetyl-CoA synthetase and 5'-nucleotidase, as well as the levels of adenosine and the pool of AMP+ADP were raised in cerebral cortex of ethanol rats. The synaptosomal level of adenosine was higher in control rats. Acetate caused a 3-fold increase of extrasynaptosomal adenosine level. Synaptosomes from ethanol rats showed higher rates of Ca-dependent releases of acetylcholine. 2-chloradenosine resulted in inhibition of synaptic acetylcholine release only in control rats. CONCLUSIONS: Ethanol causes an increase of acetate in brain and adenosine level in cerebral cortex. Acetate causes an increase of the extrasynaptic adenosine level. Prolonged ethanol treatment results in an increase of synaptic Ca-dependent acetylcholine release. Seven-day treatment with ethanol eliminates the inhibitory effect of 2-chloroadenosine-A1 adenosine receptor agonist on synaptic Ca-dependent acetylcholine release.
BACKGROUND: The aim of this study was to estimate the role of acetate-induced metabolic changes in brain mechanisms of resistance to the narcotic effect of ethanol. MATERIAL/ METHODS:Wistar rats were treated daily with ethanol (3.5 g/kg i.p. for 7 days). During alcohol treatment, the duration of ethanol-induced sleep was decreased. Levels of acetate, acetyl-CoA, adenosine, and the pool of AMP+ADP, and the activity of acetyl-CoA synthetase and 5'-nucleotidase in the brain were measured. Synaptosomal adenosine and acetylcholine release were measured in the presence of acetate, adenosine and 2-chloroadenosine. RESULTS: The concentration of acetate was higher in all investigated brain regions of ethanolrats in comparison to controls. The activity of acetyl-CoA synthetase and 5'-nucleotidase, as well as the levels of adenosine and the pool of AMP+ADP were raised in cerebral cortex of ethanolrats. The synaptosomal level of adenosine was higher in control rats. Acetate caused a 3-fold increase of extrasynaptosomal adenosine level. Synaptosomes from ethanolrats showed higher rates of Ca-dependent releases of acetylcholine. 2-chloradenosine resulted in inhibition of synaptic acetylcholine release only in control rats. CONCLUSIONS:Ethanol causes an increase of acetate in brain and adenosine level in cerebral cortex. Acetate causes an increase of the extrasynaptic adenosine level. Prolonged ethanol treatment results in an increase of synaptic Ca-dependent acetylcholine release. Seven-day treatment with ethanol eliminates the inhibitory effect of 2-chloroadenosine-A1 adenosine receptor agonist on synaptic Ca-dependent acetylcholine release.
Authors: Lihong Jiang; Barbara Irene Gulanski; Henk M De Feyter; Stuart A Weinzimer; Brian Pittman; Elizabeth Guidone; Julia Koretski; Susan Harman; Ismene L Petrakis; John H Krystal; Graeme F Mason Journal: J Clin Invest Date: 2013-03-08 Impact factor: 14.808
Authors: Ingrid D Schweigert; Cintia Roehrig; Fabiane da Costa; Fernando Scheibel; Carmen J Silveria Gottfried; Liane N Rotta; Carlos Alberto Gonçalves; Diogo O Souza; Marcos L S Perry Journal: Neurochem Res Date: 2004-08 Impact factor: 3.996
Authors: Jie Wang; Hongying Du; Xiaoxian Ma; Brian Pittman; Laura Castracane; Ting-Kai Li; Kevin L Behar; Graeme F Mason Journal: J Neurochem Date: 2013-09-23 Impact factor: 5.372
Authors: W A García-Suástegui; L A Ramos-Chávez; M Rubio-Osornio; M Calvillo-Velasco; J A Atzin-Méndez; J Guevara; D Silva-Adaya Journal: Oxid Med Cell Longev Date: 2017-01-10 Impact factor: 6.543
Authors: Jody Tanabe; Sarah Neff; Brianne Sutton; Sam Ellis; Luke Patten; Mark S Brown; Paula L Hoffman; Boris Tabakoff; Ellen L Burnham Journal: Alcohol Clin Exp Res Date: 2021-04-16 Impact factor: 3.455
Authors: Marta Pardo; Adrienne J Betz; Noemí San Miguel; Laura López-Cruz; John D Salamone; Mercè Correa Journal: Front Behav Neurosci Date: 2013-07-10 Impact factor: 3.558