| Literature DB >> 12754522 |
Yunhong Zan1, Jill D Haag, Kai-Shun Chen, Laurie A Shepel, Don Wigington, Yu-Rong Wang, Rong Hu, Christine C Lopez-Guajardo, Heidi L Brose, Katherine I Porter, Rachel A Leonard, Andrew A Hitt, Stacy L Schommer, Anu F Elegbede, Michael N Gould.
Abstract
The rat is a widely used model in biomedical research and is often the preferred rodent model in many areas of physiological and pathobiological research. Although many genetic tools are available for the rat, methods to produce gene-disrupted knockout rats are greatly needed. In this study, we developed protocols for creating N-ethyl-N-nitrosourea (ENU)-induced germline mutations in several rat strains. F1 preweanling pups from mutagenized Sprague Dawley (SD) male rats were then screened for functional mutations in Brca1 and Brca2 using a yeast gap-repair, ADE2-reporter truncation assay. We produced knockout rats for each of these two breast cancer suppressor genes.Entities:
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Year: 2003 PMID： 12754522 DOI： 10.1038/nbt830
Source DB: PubMed Journal: Nat Biotechnol ISSN： 1087-0156 Impact factor: 54.908