R Cuero1, T Ouellet, J Yu, N Mogongwa. 1. Prairie View A & M University, CARC, PO BOX 685, Prairie View, TX 77446, USA. olimpa@aol.com
Abstract
AIMS: To determine the effect of mineral ions (e.g., Zn2+, Cu2+, and Fe2+) on the enhancement of fungal growth, total RNA, aflatoxin pathway gene expression, and production of aflatoxin and its precursor O-methylsterigmatocystin (OMST). METHODS AND RESULTS: The influence of the metal ions, as a single or mixed treatments, was observed in submerged cultures of toxigenic Aspergillus flavus through changes in the fungal RNA or aflatoxin pathway gene (omtA) by reverse transcription-polymerase chain reaction (RT-PCR) levels, and also in fungal dry-weight accumulation, aflatoxin and OMST production. CONCLUSIONS: The ion treatments induced changes of fungal total RNA, mRNA levels, associate fungal growth, biosynthesis of aflatoxin and OMST, and enhanced expression of RT-PCR. SIGNIFICANCE AND IMPACT OF STUDY: Demonstrates at the cellular and molecular level, the significant effects of metal ions on both fungal growth and production of aflatoxin.
AIMS: To determine the effect of mineral ions (e.g., Zn2+, Cu2+, and Fe2+) on the enhancement of fungal growth, total RNA, aflatoxin pathway gene expression, and production of aflatoxin and its precursor O-methylsterigmatocystin (OMST). METHODS AND RESULTS: The influence of the metal ions, as a single or mixed treatments, was observed in submerged cultures of toxigenic Aspergillus flavus through changes in the fungal RNA or aflatoxin pathway gene (omtA) by reverse transcription-polymerase chain reaction (RT-PCR) levels, and also in fungal dry-weight accumulation, aflatoxin and OMST production. CONCLUSIONS: The ion treatments induced changes of fungal total RNA, mRNA levels, associate fungal growth, biosynthesis of aflatoxin and OMST, and enhanced expression of RT-PCR. SIGNIFICANCE AND IMPACT OF STUDY: Demonstrates at the cellular and molecular level, the significant effects of metal ions on both fungal growth and production of aflatoxin.
Authors: Laetitia Pinson-Gadais; Florence Richard-Forget; Pierre Frasse; Christian Barreau; Bernard Cahagnier; Daniel Richard-Molard; Bénédicte Bakan Journal: Mycopathologia Date: 2007-10-30 Impact factor: 2.574