BACKGROUND: Helicobacter pylori is an important pathogen responsible for gastroduodenal diseases in humans. Although the eradication of H. pylori using antibiotics often improves gastroduodenal diseases, resistance to the antibiotics is emerging. MATERIALS AND METHODS: The antimicrobial effect of essential oils and the development of resistance to the essential oils were evaluated in vitro and in vivo. RESULTS: Thirteen essential oils used in this study completely inhibited the growth of H. pylori in vitro at a concentration of 0.1% (v/v). Cymbopogon citratus (lemongrass) and Lippia citriodora (lemon verbena) were bactericidal against H. pylori at 0.01% at pH 4.0 and 5.0. Resistance to lemongrass did not develop even after 10 sequential passages, whereas resistance to clarithromycin developed under the same conditions. In in vivo studies, the density of H. pylori in the stomach of mice treated with lemongrass was significantly reduced compared with untreated mice. CONCLUSIONS: These results demonstrate that the essential oils are bactericidal against H. pylori without the development of acquired resistance, suggesting that essential oils may have potential as new and safe agents for inclusion in anti-H. pylori regimens.
BACKGROUND:Helicobacter pylori is an important pathogen responsible for gastroduodenal diseases in humans. Although the eradication of H. pylori using antibiotics often improves gastroduodenal diseases, resistance to the antibiotics is emerging. MATERIALS AND METHODS: The antimicrobial effect of essential oils and the development of resistance to the essential oils were evaluated in vitro and in vivo. RESULTS: Thirteen essential oils used in this study completely inhibited the growth of H. pylori in vitro at a concentration of 0.1% (v/v). Cymbopogon citratus (lemongrass) and Lippia citriodora (lemon verbena) were bactericidal against H. pylori at 0.01% at pH 4.0 and 5.0. Resistance to lemongrass did not develop even after 10 sequential passages, whereas resistance to clarithromycin developed under the same conditions. In in vivo studies, the density of H. pylori in the stomach of mice treated with lemongrass was significantly reduced compared with untreated mice. CONCLUSIONS: These results demonstrate that the essential oils are bactericidal against H. pylori without the development of acquired resistance, suggesting that essential oils may have potential as new and safe agents for inclusion in anti-H. pylori regimens.
Authors: Raphaelle Sousa Borges; Hady Keita; Brenda Lorena Sánchez Ortiz; Tafnis Ingret Dos Santos Sampaio; Irlon Maciel Ferreira; Emerson Silva Lima; Márcia de Jesus Amazonas da Silva; Caio Pinho Fernandes; Anna Eliza Maciel de Faria Mota Oliveira; Edemilson Cardoso da Conceição; Alex Bruno Lobato Rodrigues; Arlindo César Matias Pereira Filho; Andrés Navarrete Castro; José Carlos Tavares Carvalho Journal: Inflammopharmacology Date: 2018-02-05 Impact factor: 4.473
Authors: Eyad M Salem; Talay Yar; Abdullah O Bamosa; Abdulaziz Al-Quorain; Mohamed I Yasawy; Raed M Alsulaiman; Muhammad A Randhawa Journal: Saudi J Gastroenterol Date: 2010 Jul-Sep Impact factor: 2.485
Authors: Ramzi A A Mothana; Salah A A Abdo; Sidgi Hasson; Faisal M N Althawab; Sama A Z Alaghbari; Ulrike Lindequist Journal: Evid Based Complement Alternat Med Date: 2008-01-28 Impact factor: 2.629