AIM: To investigate the effect of pro-inflammatory cytokines and black-pigmented Bacteroides on the expression of IL-6 gene in human pulp fibroblasts. METHODOLOGY: IL-1alpha, tumour necrosis factor-alpha (TNF-alpha) and the supernatants of Porphyromonas endodontalis, P. gingivalis and Prevotella intermedia were used to evaluate IL-6 gene expression in human pulp fibroblasts. The levels of mRNAs were measured by the quantitative reverse-transcriptase polymerase chain reaction analysis. RESULTS: Investigations of the time dependence of IL-6 mRNA expression in pro-inflammatory cytokines-treated cells revealed a rapid accumulation of the transcript after 2 h of exposure and remained elevated throughout the 24-h incubation period. In addition, black-pigmented Bacteroides also induced IL-6 gene expression in human pulp fibroblasts. CONCLUSIONS: Pro-inflammatory cytokines and black-pigmented Bacteroides may be involved in developing pulpal inflammation through the stimulation of IL-6 production.
AIM: To investigate the effect of pro-inflammatory cytokines and black-pigmented Bacteroides on the expression of IL-6 gene in human pulp fibroblasts. METHODOLOGY: IL-1alpha, tumour necrosis factor-alpha (TNF-alpha) and the supernatants of Porphyromonas endodontalis, P. gingivalis and Prevotella intermedia were used to evaluate IL-6 gene expression in human pulp fibroblasts. The levels of mRNAs were measured by the quantitative reverse-transcriptase polymerase chain reaction analysis. RESULTS: Investigations of the time dependence of IL-6 mRNA expression in pro-inflammatory cytokines-treated cells revealed a rapid accumulation of the transcript after 2 h of exposure and remained elevated throughout the 24-h incubation period. In addition, black-pigmented Bacteroides also induced IL-6 gene expression in human pulp fibroblasts. CONCLUSIONS: Pro-inflammatory cytokines and black-pigmented Bacteroides may be involved in developing pulpal inflammation through the stimulation of IL-6 production.
Authors: Reuben H Kim; Drake W Williams; Susan Bae; Rachel S Lee; Ju-Eun Oh; Shebli Mehrazarin; Tony Kim; Ki-Hyuk Shin; No-Hee Park; Mo K Kang Journal: J Endod Date: 2012-10-24 Impact factor: 4.171
Authors: Karin Cristina da Silva Modena; Leslie Caroll Casas-Apayco; Maria Teresa Atta; Carlos Alberto de Souza Costa; Josimeri Hebling; Carla Renata Sipert; Maria Fidela de Lima Navarro; Carlos Ferreira Santos Journal: J Appl Oral Sci Date: 2009 Nov-Dec Impact factor: 2.698
Authors: Carla Renata Sipert; Ana Carolina de Faria Morandini; Karin Cristina da Silva Modena; Thiago José Dionísio; Maria Aparecida Andrade Moreira Machado; Sandra Helena Penha de Oliveira; Ana Paula Campanelli; Carlos Ferreira Santos Journal: J Appl Oral Sci Date: 2013 Mar-Apr Impact factor: 2.698