| Literature DB >> 12751317 |
Marize P Miagostovich1, Patrícia C Sequeira, Flávia B Dos Santos, Amanda Maia, Rita Maria R Nogueira, Hermann G Schatzmayr, Eva Harris, Lee W Riley.
Abstract
Strain typing is a critical tool for molecular epidemiological analysis and can provide important information about the spread of dengue viruses. Here, we performed a molecular characterization of DEN-2 viruses isolated in Brazil during 1990-2000 from geographically and temporally distinct areas in order to investigate the genetic distribution of this serotype circulating in the country. Restriction site-specific polymerase chain reaction (RSS)-PCR presented the same pattern for all 52 Brazilian samples, showing the circulation of just one DEN-2 variant. Phylogenetic analysis using progressive pairwise alignments from 240-nucleotide sequences of the E/NS1 junction in 15 isolates showed that they belong to genotype III (Jamaica genotype).Entities:
Mesh:
Substances:
Year: 2003 PMID: 12751317 DOI: 10.1590/s0036-46652003000100004
Source DB: PubMed Journal: Rev Inst Med Trop Sao Paulo ISSN: 0036-4665 Impact factor: 1.846