BACKGROUND/AIMS: The Fas-mediated apoptosis pathway has been implicated in liver diseases. The aim of the study was to investigate the role of this system in alcoholic hepatitis. METHODOLOGY: The expression of Fas, Fas ligand, and intracellular signaling molecules for apoptosis were determined by immunoblot analysis in fresh frozen liver samples from 19 patients with alcoholic liver disease. RESULTS: Fas and Fas ligand expression was significantly increased in the liver of patients with alcoholic hepatitis (n = 11) as compared with alcoholic liver disease patients without hepatitis (n = 8). Similarly, there were significant differences in the expression of FADD, ICE, and CPP32 in the liver between the two groups. There were significant positive correlations between the Fas ligand and the FADD, ICE, or CPP32 levels in the liver. The expression of Fas, Fas ligand, FADD, ICE, or CPP32 correlated with serum markers of hepatic injury. Plasma soluble Fas levels in patients with alcoholic hepatitis (median: 15.1 U/mL, range: 9.7-19.2 U/mL) were significantly higher than in normal controls (n = 9) (median: 2.8 U/mL, range: 1.9-3.7 U/mL; p < 0.001). There was a significant positive correlation between plasma soluble Fas levels and the hepatic expression of FADD in these patients. CONCLUSIONS: These results indicate that Fas-mediated apoptosis may play an important role in alcoholic hepatitis.
BACKGROUND/AIMS: The Fas-mediated apoptosis pathway has been implicated in liver diseases. The aim of the study was to investigate the role of this system in alcoholic hepatitis. METHODOLOGY: The expression of Fas, Fas ligand, and intracellular signaling molecules for apoptosis were determined by immunoblot analysis in fresh frozen liver samples from 19 patients with alcoholic liver disease. RESULTS:Fas and Fas ligand expression was significantly increased in the liver of patients with alcoholic hepatitis (n = 11) as compared with alcoholic liver diseasepatients without hepatitis (n = 8). Similarly, there were significant differences in the expression of FADD, ICE, and CPP32 in the liver between the two groups. There were significant positive correlations between the Fas ligand and the FADD, ICE, or CPP32 levels in the liver. The expression of Fas, Fas ligand, FADD, ICE, or CPP32 correlated with serum markers of hepatic injury. Plasma soluble Fas levels in patients with alcoholic hepatitis (median: 15.1 U/mL, range: 9.7-19.2 U/mL) were significantly higher than in normal controls (n = 9) (median: 2.8 U/mL, range: 1.9-3.7 U/mL; p < 0.001). There was a significant positive correlation between plasma soluble Fas levels and the hepatic expression of FADD in these patients. CONCLUSIONS: These results indicate that Fas-mediated apoptosis may play an important role in alcoholic hepatitis.
Authors: Silvia Affò; Marlene Dominguez; Juan José Lozano; Pau Sancho-Bru; Daniel Rodrigo-Torres; Oriol Morales-Ibanez; Montserrat Moreno; Cristina Millán; Aurora Loaeza-del-Castillo; José Altamirano; Juan Carlos García-Pagán; Vicente Arroyo; Pere Ginès; Juan Caballería; Robert F Schwabe; Ramon Bataller Journal: Gut Date: 2012-05-25 Impact factor: 23.059
Authors: Anna Rutherford; Lindsay Y King; Linda S Hynan; Chetan Vedvyas; Wenyu Lin; William M Lee; Raymond T Chung Journal: Gastroenterology Date: 2012-08-08 Impact factor: 22.682
Authors: Nan Jiang; Xusheng Zhang; Xiufen Zheng; Di Chen; Kingsun Siu; Hongmei Wang; Thomas E Ichim; Douglas Quan; Vivian McAlister; Guihua Chen; Wei-Ping Min Journal: PLoS One Date: 2012-09-06 Impact factor: 3.240
Authors: Patricia Castillo-Dela Cruz; Alanna G Wanek; Pawan Kumar; Xiaojing An; Waleed Elsegeiny; William Horne; Adam Fitch; Ansen H P Burr; Kathyayini P Gopalakrishna; Kong Chen; Barbara A Methé; Scott W Canna; Timothy W Hand; Jay K Kolls Journal: Cell Rep Date: 2019-11-19 Impact factor: 9.423
Authors: Fengjie Hao; Francisco Javier Cubero; Pierluigi Ramadori; Lijun Liao; Ute Haas; Daniela Lambertz; Roland Sonntag; Jörg-Martin Bangen; Nikolaus Gassler; Mareike Hoss; Konrad L Streetz; Johanna Reissing; Henning W Zimmermann; Christian Trautwein; Christian Liedtke; Yulia A Nevzorova Journal: Cell Death Dis Date: 2017-10-26 Impact factor: 8.469