| Literature DB >> 12749024 |
Katarina Sandgren1, Zhong Lin, Asa Fex Svenningsen, Eva Ekblad.
Abstract
Several motility disorders originate in the enteric nervous system (ENS). Our knowledge of factors governing survival of the ENS is poor. Changes in the expression of vasoactive intestinal peptide (VIP) and nitric oxide synthase (NOS) in enteric neurons occur after neuronal injury and in intestinal adaptation. The aim of this study was to evaluate whether VIP and nitric oxide (NO) influence survival of cultured, dissociated myenteric neurons. Neuronal survival was evaluated after 0, 4, and 8 days in culture. Influence of VIP and NO on neuronal survival was examined after culturing in the presence of VIP, NO donor, VIP antiserum, or NOS inhibitor. A marked loss of neurons was noted during culturing. VIP and NO significantly promoted neuronal survival. Corroborating this was the finding of an enhanced neuronal cell loss when cultures were grown in the presence of VIP antiserum or NOS inhibitor. Copyright 2003 Wiley-Liss, Inc.Entities:
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Year: 2003 PMID： 12749024 DOI： 10.1002/jnr.10612
Source DB: PubMed Journal: J Neurosci Res ISSN： 0360-4012 Impact factor: 4.164