BACKGROUND: The database of human Expressed Sequence Tags was previously used to identify PRAC (Prostate 47:125-131, 2001), a novel gene specifically expressed in human prostate, prostate cancer, rectum, and distal colon. In this report, we have identified PRAC2, another gene with a similar expression pattern that is located adjacent to the original PRAC gene on chromosome 17q21.3. METHODS: Using a computer-based analysis, a cluster of sequence homologous ESTs was identified that is mainly derived from human prostate cDNA libraries. The tissue specificity was examined by multiple tissue RNA dot blots and RT-PCR. The PRAC2 transcript and protein were identified using Northern blot analysis, RACE-PCR, primer extension, and Western blots. RESULTS: PRAC2 encodes a 564 nucleotide RNA found in prostate, rectum, distal colon, and testis. Weak expression was also found in placenta, peripheral blood leukocytes, skin, and in two prostate cancer cell lines: LNCaP and PC-3. The transcript seems to encode a 10.5-kDa nuclear protein. The PRAC2 gene is located on chromosome 17 at position 17q21, between the Hoxb-13 gene and the recently discovered PRAC gene. CONCLUSIONS: Because of the higher expression of PRAC2 in prostate and its proximity to Hoxb-13, PRAC2 may have a function in prostate growth and development. Copyright 2003 Wiley-Liss, Inc.
BACKGROUND: The database of human Expressed Sequence Tags was previously used to identify PRAC (Prostate 47:125-131, 2001), a novel gene specifically expressed in human prostate, prostate cancer, rectum, and distal colon. In this report, we have identified PRAC2, another gene with a similar expression pattern that is located adjacent to the original PRAC gene on chromosome 17q21.3. METHODS: Using a computer-based analysis, a cluster of sequence homologous ESTs was identified that is mainly derived from human prostate cDNA libraries. The tissue specificity was examined by multiple tissue RNA dot blots and RT-PCR. The PRAC2 transcript and protein were identified using Northern blot analysis, RACE-PCR, primer extension, and Western blots. RESULTS:PRAC2 encodes a 564 nucleotide RNA found in prostate, rectum, distal colon, and testis. Weak expression was also found in placenta, peripheral blood leukocytes, skin, and in two prostate cancer cell lines: LNCaP and PC-3. The transcript seems to encode a 10.5-kDa nuclear protein. The PRAC2 gene is located on chromosome 17 at position 17q21, between the Hoxb-13 gene and the recently discovered PRAC gene. CONCLUSIONS: Because of the higher expression of PRAC2 in prostate and its proximity to Hoxb-13, PRAC2 may have a function in prostate growth and development. Copyright 2003 Wiley-Liss, Inc.
Authors: Devin C Koestler; Jing Li; John A Baron; Gregory J Tsongalis; Lynn F Butterly; Martha Goodrich; Corina Lesseur; Margaret R Karagas; Carmen J Marsit; Jason H Moore; Angeline S Andrew; Amitabh Srivastava Journal: Mod Pathol Date: 2013-07-19 Impact factor: 7.842
Authors: S Edwards; C Campbell; P Flohr; J Shipley; I Giddings; R Te-Poele; A Dodson; C Foster; J Clark; S Jhavar; G Kovacs; C S Cooper Journal: Br J Cancer Date: 2005-01-31 Impact factor: 7.640
Authors: Elizabeth J Perlman; Samantha Gadd; Stefan T Arold; Anand Radhakrishnan; Daniela S Gerhard; Lawrence Jennings; Vicki Huff; Jaime M Guidry Auvil; Tanja M Davidsen; Jeffrey S Dome; Daoud Meerzaman; Chih Hao Hsu; Cu Nguyen; James Anderson; Yussanne Ma; Andrew J Mungall; Richard A Moore; Marco A Marra; Charles G Mullighan; Jing Ma; David A Wheeler; Oliver A Hampton; Julie M Gastier-Foster; Nicole Ross; Malcolm A Smith Journal: Nat Commun Date: 2015-12-04 Impact factor: 14.919