BACKGROUND: Spontaneous metastasis following implantation at the orthotopic site is a highly desired feature in prostate cancer (CaP) models, since it would enable studies of mechanisms associated with tumor cell dissemination. METHODS: LuCaP 23.8 and LuCaP 35, hormone-sensitive CaP xenografts that express PSA and the wild-type androgen receptor, were grown orthotopically in SCID mice. When tumor volumes reached approximately 250-500 mg, primary tumors were removed to allow micrometastases to grow. RESULTS: Using this procedure we generated macroscopic metastases (>20 mg) in 71% (LuCaP 23.8) and 100% (LuCaP 35) of animals, respectively. CONCLUSIONS: These models may be used to evaluate new treatment modalities and study mechanisms associated with development of metastases. Copyright 2003 Wiley-Liss, Inc.
BACKGROUND: Spontaneous metastasis following implantation at the orthotopic site is a highly desired feature in prostate cancer (CaP) models, since it would enable studies of mechanisms associated with tumor cell dissemination. METHODS: LuCaP 23.8 and LuCaP 35, hormone-sensitive CaP xenografts that express PSA and the wild-type androgen receptor, were grown orthotopically in SCIDmice. When tumor volumes reached approximately 250-500 mg, primary tumors were removed to allow micrometastases to grow. RESULTS: Using this procedure we generated macroscopic metastases (>20 mg) in 71% (LuCaP 23.8) and 100% (LuCaP 35) of animals, respectively. CONCLUSIONS: These models may be used to evaluate new treatment modalities and study mechanisms associated with development of metastases. Copyright 2003 Wiley-Liss, Inc.
Authors: Shihua Sun; Cynthia C T Sprenger; Robert L Vessella; Kathleen Haugk; Kathryn Soriano; Elahe A Mostaghel; Stephanie T Page; Ilsa M Coleman; Holly M Nguyen; Huiying Sun; Peter S Nelson; Stephen R Plymate Journal: J Clin Invest Date: 2010-07-19 Impact factor: 14.808
Authors: Akash Kumar; Thomas A White; Alexandra P MacKenzie; Nigel Clegg; Choli Lee; Ruth F Dumpit; Ilsa Coleman; Sarah B Ng; Stephen J Salipante; Mark J Rieder; Deborah A Nickerson; Eva Corey; Paul H Lange; Colm Morrissey; Robert L Vessella; Peter S Nelson; Jay Shendure Journal: Proc Natl Acad Sci U S A Date: 2011-09-26 Impact factor: 11.205
Authors: Manish Kohli; Liguo Wang; Fang Xie; Hugues Sicotte; Ping Yin; Scott M Dehm; Steven N Hart; Peter T Vedell; Poulami Barman; Rui Qin; Douglas W Mahoney; Rachel E Carlson; Jeanette E Eckel-Passow; Thomas D Atwell; Patrick W Eiken; Brendan P McMenomy; Eric D Wieben; Gautam Jha; Rafael E Jimenez; Richard Weinshilboum; Liewei Wang Journal: PLoS One Date: 2015-12-22 Impact factor: 3.240