| Literature DB >> 12742129 |
Jörg Mühling1, Markus Fuchs, Marie E Campos, Jens Gonter, Jörg M Engel, Armin Sablotzki, Thilo Menges, Stefan Weiss, Marius G Dehne, Matthias Krüll, Gunter Hempelmann.
Abstract
For the first time, a procedure is described for the quantitative analysis of free alpha-keto acid content in human neutrophils (PMNs) relative to single cell number by reversed-phase fluorescence high-performance liquid chromatography. The procedure is minimally invasive and is unsurpassed in the quality of PMN separation, ease of sample preparation as well as sample stability. This method can satisfy the rigorous demands for an ultra-sensitive, comprehensive and rapid intracellular alpha-keto acid analysis in particularly for the surveillance of severe diseases as well as cellular or organ dysfunction.Entities:
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Year: 2003 PMID： 12742129 DOI： 10.1016/s1570-0232(03)00163-6
Source DB: PubMed Journal: J Chromatogr B Analyt Technol Biomed Life Sci ISSN： 1570-0232 Impact factor: 3.205