Literature DB >> 12735701

Comparison of the standard AGID test and competitive ELISA for detecting bluetongue virus antibodies in camels in Gujarat, India.

B S Chandel1, H C Chauhan, H N Kher.   

Abstract

The performance of the standard agar gel immunodiffusion (AGID) test and the competitive enzyme-linked immunosorbent assay (cELISA) for the detection of serum antibody against bluetongue virus (BTV) in clinically healthy and diseased camels in Gujarat state were compared. Out of 176 sera tested, 22 (12.5%) and 34 (19.3%) were positive for group-specific bluetongue antibodies by AGID and cELISA, respectively. Maximum seropositivities of 18.0% by AGID and 25.8% by cELISA were recorded in the Kutchhi breed, and of 6.9% and 12.6%, respectively, in the Marwari breed. The seroprevalence detected by AGID and cELISA in clinically healthy and diseased camels did not differ significantly with regard to bluetongue disease in these breeds.

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Year:  2003        PMID： 12735701     DOI： 10.1023/a:1022896117122

Source DB:  PubMed          Journal:  Trop Anim Health Prod        ISSN： 0049-4747            Impact factor:   1.559


  5 in total

1.  Comparison of competitive and indirect enzyme-linked immunosorbent assays for detection of bluetongue virus antibodies in serum and whole blood.

Authors:  A Afshar; F C Thomas; P F Wright; J L Shapiro; P T Shettigara; J Anderson
Journal:  J Clin Microbiol       Date:  1987-09       Impact factor: 5.948

2.  Problems in the interpretation of diagnostic tests due to cross-reactions between orbiviruses and broad serological responses in animals.

Authors:  A J Della-Porta; I M Parsonson; D A McPhee
Journal:  Prog Clin Biol Res       Date:  1985

3.  Comparison of competitive ELISA, indirect ELISA and standard AGID tests for detecting blue-tongue virus antibodies in cattle and sheep.

Authors:  A Afshar; F C Thomas; P F Wright; J L Shapiro; J Anderson
Journal:  Vet Rec       Date:  1989-02-11       Impact factor: 2.695

4.  Comparison of blocking dot ELISA and competitive ELISA, using a monoclonal antibody for detection of bluetongue virus antibodies in cattle.

Authors:  A Afshar; G C Dulac; J Riva
Journal:  Vet Microbiol       Date:  1992-04       Impact factor: 3.293

5.  Use of monoclonal antibody in a blocking ELISA to detect group specific antibodies to bluetongue virus.

Authors:  J Anderson
Journal:  J Immunol Methods       Date:  1984-11-16       Impact factor: 2.303
  5 in total
  2 in total

1.  Preparation and Characterization of a Monoclonal Antibody Against the Core Protein VP7 of the 25th Serotype of Bluetongue Virus.

Authors:  Xiao Wu; Qi Liu; Jia He; Mingxin Zang; Haixiu Wang; Yijing Li; Lijie Tang
Journal:  Monoclon Antib Immunodiagn Immunother       Date:  2015-04

2.  An updated review on bluetongue virus: epidemiology, pathobiology, and advances in diagnosis and control with special reference to India.

Authors:  Mani Saminathan; Karam Pal Singh; Jaynudin Hajibhai Khorajiya; Murali Dinesh; Sobharani Vineetha; Madhulina Maity; At Faslu Rahman; Jyoti Misri; Yashpal Singh Malik; Vivek Kumar Gupta; Raj Kumar Singh; Kuldeep Dhama
Journal:  Vet Q       Date:  2020-12       Impact factor: 3.320
  2 in total

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