| Literature DB >> 1273518 |
S Ahlstedt, L A Hanson, C Wadsworth.
Abstract
A new sensitive technique measures C1q-binding of human IgG aggregates. The method is based on the principle of the enzyme-linked immunosorbent assay with C1q-coated tubes. The IgG aggregates attaching to this C1q are shown by enzyme-linked anti-human IgG. Less than 0.01 mug of aggregates per milliliter of sample can be detected. The results with this new method showed significant correlation (P less than 0.01; Spearman rank correlation test) with the estimates of IgG aggregates of 13S or more and 10S in size obtained by thin-layer gel filtration. Both of these methods showed significant correlation with the classic hemolysis inhibition method for measuring complement fixation.Entities:
Mesh：
Substances：
Year: 1976 PMID： 1273518 DOI： 10.1111/j.1365-3083.1976.tb00281.x
Source DB: PubMed Journal: Scand J Immunol ISSN： 0300-9475 Impact factor: 3.487