Literature DB >> 12734272

Pilot study of COBAS PCR and ligase chain reaction for detection of rectal infections due to Chlamydia trachomatis.

Matthew R Golden1, Sabina G Astete, Rosa Galvan, Aldo Lucchetti, Jorge Sanchez, Connie L Celum, William L H Whittington, Walter E Stamm, King K Holmes, Patricia A Totten.   

Abstract

We tested rectal specimens from men who have sex with men for Chlamydia trachomatis by using COBAS PCR (Roche Diagnostics) and ligase chain reaction LCR (Abbott laboratories) and compared three PCR specimen-processing procedures. Chlamydiae were detected by one or more procedures in 22 of 186 specimens. All three PCR tests were positive for 17 specimens, all of which also tested positive by LCR.

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Year:  2003        PMID： 12734272      PMCID： PMC154739          DOI： 10.1128/JCM.41.5.2174-2175.2003

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  8 in total

1.  Evaluation of laboratory testing methods for Chlamydia trachomatis infection in the era of nucleic acid amplification.

Authors:  T J Battle; M R Golden; K L Suchland; J M Counts; J P Hughes; W E Stamm; K K Holmes
Journal:  J Clin Microbiol       Date:  2001-08       Impact factor: 5.948

2.  Sexually transmitted disease and hiv screening guidelines for men who have sex with men.

Authors: 
Journal:  Sex Transm Dis       Date:  2001-08       Impact factor: 2.830

3.  Guidance for STD clinical preventive services for persons infected with HIV.

Authors: 
Journal:  Sex Transm Dis       Date:  2001-08       Impact factor: 2.830

4.  PCR for detection of Chlamydia trachomatis in endocervical, urethral, rectal, and pharyngeal swab samples obtained from patients attending an STD clinic.

Authors:  L Ostergaard; T Agner; E Krarup; U B Johansen; K Weismann; E Gutschik
Journal:  Genitourin Med       Date:  1997-12

5.  Epidemiology of anorectal chlamydial and gonococcal infections among men having sex with men in Seattle: utilizing serovar and auxotype strain typing.

Authors:  William M Geisler; William L H Whittington; Robert J Suchland; Walter E Stamm
Journal:  Sex Transm Dis       Date:  2002-04       Impact factor: 2.830

6.  Detection of Chlamydia (Bedsonia) in certain infections of man. I. Laboratory procedures: comparison of yolk sac and cell culture for detection and isolation.

Authors:  F B Gordon; I A Harper; A L Quan; J D Treharne; R S Dwyer; J A Garland
Journal:  J Infect Dis       Date:  1969-10       Impact factor: 5.226

7.  Chlamydial infection in homosexual men. Frequency of isolation of Chlamydia trachomatis from the urethra, ano-rectum, and pharynx.

Authors:  A McMillan; R G Sommerville; P M McKie
Journal:  Br J Vener Dis       Date:  1981-02

8.  Sexually transmitted diseases treatment guidelines 2002. Centers for Disease Control and Prevention.

Authors: 
Journal:  MMWR Recomm Rep       Date:  2002-05-10
  8 in total
  3 in total

Review 1.  Sexually transmitted proctitis.

Authors:  E Hamlyn; C Taylor
Journal:  Postgrad Med J       Date:  2006-11       Impact factor: 2.401

2.  Bacterial sexually transmitted diseases.

Authors:  Charles B Whitlow
Journal:  Clin Colon Rectal Surg       Date:  2004-11

3.  Validation of roche COBAS Amplicor assay for detection of Chlamydia trachomatis in rectal and pharyngeal specimens by an omp1 PCR assay.

Authors:  N A Lister; S N Tabrizi; C K Fairley; S Garland
Journal:  J Clin Microbiol       Date:  2004-01       Impact factor: 5.948
  3 in total

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