Literature DB >> 12732431

Application of NucliSens Basic Kit for the detection of Mycoplasma pneumoniae in respiratory specimens.

K Loens1, M Ieven, D Ursi, H Foolen, P Sillekens, H Goossens.   

Abstract

A commercially available nucleic acid sequence-based amplification (NASBA) NucliSens Basic Kit (NBK) assay for the detection of Mycoplasma pneumoniae 16S rRNA in respiratory specimens was developed and compared to standard NASBA and PCR assays previously developed in our laboratory. The specificity and sensitivity of the NBK assay was comparable to the specificity and sensitivity of the corresponding standard NASBA assay. The NBK offers standardized reagents for the development of a NASBA assay for the detection of M. pneumoniae in respiratory specimens and is easily adaptable to other amplification targets.

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Year:  2003        PMID： 12732431     DOI： 10.1016/s0167-7012(03)00011-3

Source DB:  PubMed          Journal:  J Microbiol Methods        ISSN： 0167-7012            Impact factor:   2.363


  5 in total

1.  Detection of Mycoplasma pneumoniae by real-time nucleic acid sequence-based amplification.

Authors:  K Loens; M Ieven; D Ursi; T Beck; M Overdijk; P Sillekens; H Goossens
Journal:  J Clin Microbiol       Date:  2003-09       Impact factor: 5.948

2.  Development of conventional and real-time nucleic acid sequence-based amplification assays for detection of Chlamydophila pneumoniae in respiratory specimens.

Authors:  K Loens; T Beck; H Goossens; D Ursi; M Overdijk; P Sillekens; M Ieven
Journal:  J Clin Microbiol       Date:  2006-04       Impact factor: 5.948

3.  Two quality control exercises involving nucleic acid amplification methods for detection of Mycoplasma pneumoniae and Chlamydophila pneumoniae and carried out 2 years apart (in 2002 and 2004).

Authors:  K Loens; T Beck; D Ursi; S Pattyn; H Goossens; M Ieven
Journal:  J Clin Microbiol       Date:  2006-03       Impact factor: 5.948

Review 4.  Mycoplasma pneumoniae from the Respiratory Tract and Beyond.

Authors:  Ken B Waites; Li Xiao; Yang Liu; Mitchell F Balish; T Prescott Atkinson
Journal:  Clin Microbiol Rev       Date:  2017-07       Impact factor: 26.132

5.  Development of real-time multiplex nucleic acid sequence-based amplification for detection of Mycoplasma pneumoniae, Chlamydophila pneumoniae, and Legionella spp. in respiratory specimens.

Authors:  K Loens; T Beck; D Ursi; M Overdijk; P Sillekens; H Goossens; M Ieven
Journal:  J Clin Microbiol       Date:  2007-11-21       Impact factor: 5.948
  5 in total

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