Literature DB >> 12729998

Comparison of real-time PCR detection methods for B1 and P30 genes of Toxoplasma gondii.

Susanne Buchbinder1, Rosemarie Blatz, Arne Christian Rodloff.   

Abstract

Real-time PCR assays were established with the Toxoplasma gondii P30 and the 35-copy B1 gene as target genes. Both PCR methods detected Toxoplasma DNA from 10 to 100000 genome equivalents per assay and had comparable intra-assay deviations suggesting that detecting a single copy locus is suitable for clinical diagnostic.

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Year:  2003        PMID： 12729998     DOI： 10.1016/s0732-8893(02)00549-7

Source DB:  PubMed          Journal:  Diagn Microbiol Infect Dis        ISSN： 0732-8893            Impact factor:   2.803


  17 in total

1.  Comparison of two widely used PCR primer systems for detection of toxoplasma in amniotic fluid, blood, and tissues.

Authors:  Elisabeth Chabbert; Laurence Lachaud; Lucien Crobu; Patrick Bastien
Journal:  J Clin Microbiol       Date:  2004-04       Impact factor: 5.948

2.  Comparison between two amplification sets for molecular diagnosis of toxoplasmosis by real-time PCR.

Authors:  S Cassaing; M H Bessières; A Berry; A Berrebi; R Fabre; J F Magnaval
Journal:  J Clin Microbiol       Date:  2006-03       Impact factor: 5.948

3.  Detection of Toxoplasma gondii oocysts in water sample concentrates by real-time PCR.

Authors:  Wenli Yang; H D Alan Lindquist; Vitaliano Cama; Frank W Schaefer; Eric Villegas; Ronald Fayer; Earl J Lewis; Yaoyu Feng; Lihua Xiao
Journal:  Appl Environ Microbiol       Date:  2009-04-10       Impact factor: 4.792

4.  Quantitative real-time PCR is not more sensitive than "conventional" PCR.

Authors:  Patrick Bastien; Gary W Procop; Udo Reischl
Journal:  J Clin Microbiol       Date:  2008-04-09       Impact factor: 5.948

5.  Diagnostic and prognostic value of cell free circulating Schistosoma mansoni DNA: an experimental study.

Authors:  Maysa Ahmad Eraky; Nagwa Shaban Mohamed Aly
Journal:  J Parasit Dis       Date:  2014-12-20

Review 6.  The common zoonotic protozoal diseases causing abortion.

Authors:  Raafat Mohamed Shaapan
Journal:  J Parasit Dis       Date:  2015-03-14

7.  Evaluation of a real-time PCR assay based on the repetitive B1 gene for the detection of Toxoplasma gondii in human peripheral blood.

Authors:  Alicia Kompalic-Cristo; Cassia Frotta; Martha Suárez-Mutis; Octavio Fernandes; Constança Britto
Journal:  Parasitol Res       Date:  2007-03-25       Impact factor: 2.289

8.  Use of fluorescence resonance energy transfer hybridization probes to evaluate quantitative real-time PCR for diagnosis of ocular toxoplasmosis.

Authors:  Audrey Simon; Pierre Labalette; Isabelle Ordinaire; Emilie Fréalle; Eduardo Dei-Cas; Daniel Camus; Laurence Delhaes
Journal:  J Clin Microbiol       Date:  2004-08       Impact factor: 5.948

9.  Real-time RT-PCR on SAG1 and BAG1 gene expression during stage conversion in immunosuppressed mice infected with Toxoplasma gondii Tehran strain.

Authors:  Monavar Selseleh; Mohammad Hossein Modarressi; Mehdi Mohebali; Saeedeh Shojaee; Mohammad Reza Eshragian; Mina Selseleh; Ebrahim Azizi; Hossein Keshavarz
Journal:  Korean J Parasitol       Date:  2012-08-13       Impact factor: 1.341

10.  Brain Tissue Cysts in Infected Mice with RH-Strain of Toxoplasma gondii and Evaluation of BAG1 and SAG1 Genes Expression.

Authors:  Monavar Selseleh; Mh Modarressi; S Shojaee; M Mohebali; Mr Eshraghian; Mina Selseleh; H Keshavarz
Journal:  Iran J Parasitol       Date:  2013-01       Impact factor: 1.012
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