Literature DB >> 12725935

Optimisation of interdelta analysis for Saccharomyces cerevisiae strain characterisation.

Jean-Luc Legras1, Francis Karst.   

Abstract

A new primer pair (delta12-delta21) for polymerase chain reaction-based yeast typing was designed using the yeast genome sequence. The specificity of this primer pair was checked by the comparison of the electrophoresis pattern with a virtual profile calculated from Blast data. The analysis of 53 commercial and laboratory Saccharomyces cerevisiae yeast strains showed a clear improvement of interdelta analysis using the newly designed primers.

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Year:  2003        PMID： 12725935     DOI： 10.1016/S0378-1097(03)00205-2

Source DB:  PubMed          Journal:  FEMS Microbiol Lett        ISSN： 0378-1097            Impact factor:   2.742


  60 in total

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