Literature DB >> 12725697

Separation and determination of phospholipids in biological samples by high-performance liquid chromatography.

Fengjun Wang1, Yun Zhao, Pei Wang.   

Abstract

An isocratic high-performance liquid chromatographic method is developed for the determination of phospholipids in biological samples using a muPorasil silica column and a mobile phase of acetonitrile-methanol-85% phosphoric acid (90:3:1, v/v/v) at a flow rate of 0.80 mL/min. The effluent is monitored by a UV detector at 203 nm. With the method reported in this paper, phosphatidylinostol, phosphatidylserine, phosphatidylethnolamine, and phosphatidylcholine in biological samples are separated and detected successfully. The method is simple, rapid, and has excellent precision.

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Year:  2003        PMID： 12725697     DOI： 10.1093/chromsci/41.3.142

Source DB:  PubMed          Journal:  J Chromatogr Sci        ISSN： 0021-9665            Impact factor:   1.618


  6 in total

1.  Phosphatidylserine synthesis at membrane contact sites promotes its transport out of the ER.

Authors:  Muthukumar Kannan; Sujoy Lahiri; Li-Ka Liu; Vineet Choudhary; William A Prinz
Journal:  J Lipid Res       Date:  2017-01-24       Impact factor: 5.922

2.  Nonvesicular phospholipid transfer between peroxisomes and the endoplasmic reticulum.

Authors:  Sumana Raychaudhuri; William A Prinz
Journal:  Proc Natl Acad Sci U S A       Date:  2008-10-03       Impact factor: 11.205

3.  ER-shaping proteins facilitate lipid exchange between the ER and mitochondria in S. cerevisiae.

Authors:  Christiane Voss; Sujoy Lahiri; Barry P Young; Christopher J Loewen; William A Prinz
Journal:  J Cell Sci       Date:  2012-07-13       Impact factor: 5.285

4.  A conserved endoplasmic reticulum membrane protein complex (EMC) facilitates phospholipid transfer from the ER to mitochondria.

Authors:  Sujoy Lahiri; Jesse T Chao; Shabnam Tavassoli; Andrew K O Wong; Vineet Choudhary; Barry P Young; Christopher J R Loewen; William A Prinz
Journal:  PLoS Biol       Date:  2014-10-14       Impact factor: 8.029

5.  Phosphatidylserine decarboxylase is critical for the maintenance of skeletal muscle mitochondrial integrity and muscle mass.

Authors:  Ahrathy Selathurai; Greg M Kowalski; Shaun A Mason; Damien L Callahan; Victoria C Foletta; Paul A Della Gatta; Angus Lindsay; Steven Hamley; Gunveen Kaur; Annie R Curtis; Micah L Burch; Teddy Ang; Sean L McGee; Clinton R Bruce
Journal:  Mol Metab       Date:  2019-06-27       Impact factor: 7.422

6.  Nonvesicular sterol movement from plasma membrane to ER requires oxysterol-binding protein-related proteins and phosphoinositides.

Authors:  Sumana Raychaudhuri; Young Jun Im; James H Hurley; William A Prinz
Journal:  J Cell Biol       Date:  2006-04-03       Impact factor: 10.539
  6 in total

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