PURPOSE: The present study was undertaken to determine the presence of 3 periodontopathic bacteria in the supragingival plaques of 3- to 16-year-old children with different oral health conditions. METHODS: DMFT and dft, PMA index (P=papillary gingivitis, M=marginal gingivitis, and A=attached gingivitis), OHI (oral hygiene index), and oral malodor of each subject were determined prior to the collection of supragingival plaques. Periodontopathic bacteria (P. gingivalis, P. intermedia, and A. actinomycetemcomitans) in supragingival plaques were detected using an immunoslot blot assay with monoclonal 3 periodontopathic bacteria in the 2 subject groups (children with and without caries). P. gingivalis-positive subjects, but not their P. intermedia or A. actinomycetemcomitans counterparts, were correlated to oral malodor. Oral malodor was also correlated to debris index, a component of OHI. RESULTS: The group with the higher OHI showed a higher prevalence of periodontopathic bacteria. For the 3 periodontopathic bacteria in the subjects tested, P. gingivalis-, P. intermedia-, and A. actinomycetemcomitans-positive plaques were not age related. CONCLUSIONS: The supragingival plaques in children can harbor periodontopathic bacteria such as P. gingivalis, P. intermedia, and A. actinomycetemcomitans.
PURPOSE: The present study was undertaken to determine the presence of 3 periodontopathic bacteria in the supragingival plaques of 3- to 16-year-old children with different oral health conditions. METHODS: DMFT and dft, PMA index (P=papillary gingivitis, M=marginal gingivitis, and A=attached gingivitis), OHI (oral hygiene index), and oral malodor of each subject were determined prior to the collection of supragingival plaques. Periodontopathic bacteria (P. gingivalis, P. intermedia, and A. actinomycetemcomitans) in supragingival plaques were detected using an immunoslot blot assay with monoclonal 3 periodontopathic bacteria in the 2 subject groups (children with and without caries). P. gingivalis-positive subjects, but not their P. intermedia or A. actinomycetemcomitans counterparts, were correlated to oral malodor. Oral malodor was also correlated to debris index, a component of OHI. RESULTS: The group with the higher OHI showed a higher prevalence of periodontopathic bacteria. For the 3 periodontopathic bacteria in the subjects tested, P. gingivalis-, P. intermedia-, and A. actinomycetemcomitans-positive plaques were not age related. CONCLUSIONS: The supragingival plaques in children can harbor periodontopathic bacteria such as P. gingivalis, P. intermedia, and A. actinomycetemcomitans.
Authors: M Regina Simionato; Chelsea M Tucker; Masae Kuboniwa; Gwyneth Lamont; Donald R Demuth; Gena D Tribble; Richard J Lamont Journal: Infect Immun Date: 2006-08-21 Impact factor: 3.441