Literature DB >> 12715419

[Analysis of sperm chromosomes in infertile males with teratozoospermia].

Monika A Szczygieł1, Hirokazu Kusakabe, Ewa Wiland, Maciej Kurpisz.   

Abstract

OBJECTIVES: The aim of the study was to examine sperm chromosomes from infertile males with teratozoospermia.
DESIGN: Basic research study
MATERIALS AND METHODS: Spermatozoa were obtained from infertile males with teratozoospermia. Intracytoplasmic sperm injection was used to inject human spermatozoa into mouse oocytes in order to examine sperm chromosomes.
RESULTS: Chromosomes of spermatozoa obtained from 6 infertile teratozoospermic and 1 fertile normozoospermic males were investigated. The mean frequency of structural chromosome aberrations in amorphous, motile spermatozoa was similar to that of morphologically normal sperm (9 vs. 7.7%). The structural aberrations found in amorphous, immotile spermatozoa reached 93% and revealed severity was significant.
CONCLUSIONS: Teratozoospermia per se did not increase the frequency of structural chromosome aberrations in sperm. Severe chromosomal damage was revealed when immotile spermatozoa were injected into oocytes by ICSI.

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Year:  2003        PMID: 12715419

Source DB:  PubMed          Journal:  Ginekol Pol        ISSN: 0017-0011            Impact factor:   1.232


  1 in total

1.  Short-term storage of human spermatozoa in electrolyte-free medium without freezing maintains sperm chromatin integrity better than cryopreservation.

Authors:  Jonathan M Riel; Yasuhiro Yamauchi; Thomas T F Huang; John Grove; Monika A Ward
Journal:  Biol Reprod       Date:  2011-05-18       Impact factor: 4.285

  1 in total

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