Literature DB >> 12706832

In situ observation of mobility and anchoring of PKCbetaI in plasma membrane.

Kenta Saito1, Eiko Ito, Yuichi Takakuwa, Mamoru Tamura, Masataka Kinjo.   

Abstract

We employed fluorescence correlation spectroscopy (FCS) to analyze the characteristics of biomolecules in living cells. Protein kinase C (PKC) changes its subcellular localization from cytosol to the plasma membrane by its ligand. Using FCS, we found PKCbetaI labeled with enhanced green fluorescent protein freely diffusing in cytosol. Upon 12-O-tetradecanoylphorbol-13-acetate activation, a large part of PKCbetaI is anchored in the plasma membrane but some PKCbetaI still moves freely near the plasma membrane. These results indicate that a diffusion-driven transport mechanism is appropriate for the molecular mechanism of the PKCbetaI localization change.

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Year:  2003        PMID： 12706832     DOI： 10.1016/s0014-5793(03)00324-7

Source DB:  PubMed          Journal:  FEBS Lett        ISSN： 0014-5793            Impact factor:   4.124


  10 in total

1.  Lateral mobility of membrane-binding proteins in living cells measured by total internal reflection fluorescence correlation spectroscopy.

Authors:  Yu Ohsugi; Kenta Saito; Mamoru Tamura; Masataka Kinjo
Journal:  Biophys J       Date:  2006-08-04       Impact factor: 4.033

2.  Microenvironment and effect of energy depletion in the nucleus analyzed by mobility of multiple oligomeric EGFPs.

Authors:  Changi Pack; Kenta Saito; Mamoru Tamura; Masataka Kinjo
Journal:  Biophys J       Date:  2006-09-01       Impact factor: 4.033

3.  Comparison of protein behavior between wild-type and G601S hERG in living cells by fluorescence correlation spectroscopy.

Authors:  Eri H Hayakawa; Michiko Furutani; Rumiko Matsuoka; Yuichi Takakuwa
Journal:  J Physiol Sci       Date:  2011-05-15       Impact factor: 2.781

4.  Single-molecule analyses of the dynamics of heat shock protein 104 (Hsp104) and protein aggregates.

Authors:  Momoko Okuda; Tatsuya Niwa; Hideki Taguchi
Journal:  J Biol Chem       Date:  2015-01-29       Impact factor: 5.157

Review 5.  Protein Kinase C as Regulator of Vascular Smooth Muscle Function and Potential Target in Vascular Disorders.

Authors:  H C Ringvold; R A Khalil
Journal:  Adv Pharmacol       Date:  2016-07-18

Review 6.  Evolving mechanisms of vascular smooth muscle contraction highlight key targets in vascular disease.

Authors:  Zhongwei Liu; Raouf A Khalil
Journal:  Biochem Pharmacol       Date:  2018-02-13       Impact factor: 5.858

Review 7.  Insight into intra- and inter-molecular interactions of PKC: design of specific modulators of kinase function.

Authors:  Viktoria Kheifets; Daria Mochly-Rosen
Journal:  Pharmacol Res       Date:  2007-05-03       Impact factor: 7.658

8.  Role of PKCbeta in hepatocellular carcinoma cells migration and invasion in vitro: a potential therapeutic target.

Authors:  Kun Guo; Yan Li; Xiaonan Kang; Lu Sun; Jiefeng Cui; Dongmei Gao; Yinkun Liu
Journal:  Clin Exp Metastasis       Date:  2008-12-27       Impact factor: 5.150

9.  The use of fluorescence correlation spectroscopy (FCS) as an alternative biomarker detection technique: a preliminary study.

Authors:  Aamir Shahzad; Martin Knapp; Irene Lang; Gottfried Köhler
Journal:  J Cell Mol Med       Date:  2011-12       Impact factor: 5.310

10.  A Quantitative Study of Internal and External Interactions of Homodimeric Glucocorticoid Receptor Using Fluorescence Cross-Correlation Spectroscopy in a Live Cell.

Authors:  Manisha Tiwari; Sho Oasa; Johtaro Yamamoto; Shintaro Mikuni; Masataka Kinjo
Journal:  Sci Rep       Date:  2017-06-28       Impact factor: 4.379
  10 in total

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