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Literature DB >> 1270517

DNA replication in Chinese hamster ovary cells made permeable to nucleotides by tween-80 treatment.

Abstract

We have developed a method for permeabilizing CHO cells to nucleotides under conditions which allow most cells to remain viable. Permeabilized cells can carry out ATP-dependent, semiconservative synthesis of DNA. The data are consistent with the continuation of DNA synthesis in those cells in S phase at the time of treatment, possibly limited to completion of replicon synthesis without new initiations.

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Year: 1976 PMID： 1270517 PMCID： PMC2109700 DOI： 10.1083/jcb.69.3.732

Source DB: PubMed Journal: J Cell Biol ISSN： 0021-9525 Impact factor: 10.539

11 in total

1. THE EFFECTS OF TWEEN 80 ON THE IN VITRO METABOLISM OF CELLS OF THE EHRLICH-LETTR'E ASCITES CARCINOMA.

Authors: E R KAY
Journal: Cancer Res Date: 1965-06 Impact factor: 12.701

2. Role for deoxyribonucleic acid ligase in deoxyribonucleic acid polymerase i-dependent repair synthesis in toluene-treated escherichia coli.

Authors: D Billen; G R Hellermann; D R Stallions
Journal: J Bacteriol Date: 1975-10 Impact factor: 3.490

DNA replication in Chinese hamster ovary cells made permeable to nucleotides by tween-80 treatment.

1. THE EFFECTS OF TWEEN 80 ON THE IN VITRO METABOLISM OF CELLS OF THE EHRLICH-LETTR'E ASCITES CARCINOMA.

2. Role for deoxyribonucleic acid ligase in deoxyribonucleic acid polymerase i-dependent repair synthesis in toluene-treated escherichia coli.

3. Modification of replicon operation in HeLa cells by 2,4-dinitrophenol.

4. Programmed synthesis of deoxyribonucleic acid during the cell cycle.

5. On the mechanism of DNA replication in mammalian chromosomes.

6. X-ray-induced perturbations in the replication of the bacterial chromosome.

7. A permeable cell system for studying DNA replication in synchronized HeLa cells.

8. Replication and repair of DNA in cells of Escherichia coli treated with toluene.

9. Effects of toluene on Escherichia coli.

10. DNA replication: direction and rate of chain growth in mammalian cells.

1. Permeable embryonic cells of sea urchins as a model for studying nucleus-cytoplasm interactions.

2. Organellar DNA synthesis in permeabilized soybean cells.