Feng Zhu1, Xin-Guang Liu, Nian-Ci Liang. 1. Institute of Biochemistry and Molecular Biology, Guangdong Medical College, Zhanjiang, Guangdong, PR China.
Abstract
BACKGROUND & OBJECTIVE: Emodin inhibited the activity of TPK and CK2 and the degradation of I-kappaB. Apigenin inhibited the activity of MAPK and PI(3)K. In this study the authors observed the effect of emodin and apigenin on the invasion of HO-8910PM cells in vitro. METHODS: Trypan blue dye exclusion assay was used to examine the cytotoxity of emodin and apigenin. Reconstituted basement membrane invasion assay was utilized to evaluate the invasive activity. Type IV collagenase production was analyzed by PAGE substrate zymography. RESULTS: Emodin had weaker cytotoxity on HO-8910PM cells than apigenin, their IC(50) after treatment with the chemicals for 48 hours were (35.30+/-3.50) micromol/L, and (28.92+/-2.60)micromol/L, while emodin significantly inhibited membrane invasion and adhesion and migration of HO-8910PM cells. Their inhibition rates after treated with the chemical of 40 micromol/L were (45.31+/-3.10)%,(25.42+/-1.70)%, and (41.59+/-1.90)%. Emodin inhibited the production but not activity of MMP-9. Apigenin inhibited migration and adhesion of HO-8910PM cells, their inhibition rates after treated with the chemical of 40 micromol/L were (29.04+/-1.70)% and (30.80+/-3.00)%, while weakly inhibited membrane invasion (the inhibition rate only was 12.1%) and inhibited neither production nor activity of MMP-9. CONCLUSION: Both emodin and apigenin had cytotoxicity on HO-8910PM cells. Emodin was a potential agent inhibiting tumor invasion and metastasis.
BACKGROUND & OBJECTIVE: Emodin inhibited the activity of TPK and CK2 and the degradation of I-kappaB. Apigenin inhibited the activity of MAPK and PI(3)K. In this study the authors observed the effect of emodin and apigenin on the invasion of HO-8910PM cells in vitro. METHODS:Trypan blue dye exclusion assay was used to examine the cytotoxity of emodin and apigenin. Reconstituted basement membrane invasion assay was utilized to evaluate the invasive activity. Type IV collagenase production was analyzed by PAGE substrate zymography. RESULTS: Emodin had weaker cytotoxity on HO-8910PM cells than apigenin, their IC(50) after treatment with the chemicals for 48 hours were (35.30+/-3.50) micromol/L, and (28.92+/-2.60)micromol/L, while emodin significantly inhibited membrane invasion and adhesion and migration of HO-8910PM cells. Their inhibition rates after treated with the chemical of 40 micromol/L were (45.31+/-3.10)%,(25.42+/-1.70)%, and (41.59+/-1.90)%. Emodin inhibited the production but not activity of MMP-9. Apigenin inhibited migration and adhesion of HO-8910PM cells, their inhibition rates after treated with the chemical of 40 micromol/L were (29.04+/-1.70)% and (30.80+/-3.00)%, while weakly inhibited membrane invasion (the inhibition rate only was 12.1%) and inhibited neither production nor activity of MMP-9. CONCLUSION: Both emodin and apigenin had cytotoxicity on HO-8910PM cells. Emodin was a potential agent inhibiting tumor invasion and metastasis.