OBJECTIVE: To evaluate the occurrence of free-living amoebas of the genera Acanthamoeba and Naegleria is dust samples colleted in two hospitals. METHODS: One-hundred and thirty-two dust samples were collected in two hospitals in Brazil. Hospital collection sites were the following: intensive care unit, operation rooms, nursery, kitchen, emergency and infectious diseases isolation room. The isolation of the amoebas was performed in three culture media: non-nutrient agar inoculated with Escherichia coli, soy agar, and microculture in Giazzi-modified Pavlova's medium. The amoebas were identified according to morphological criteria. RESULTS: Amoebas of the genera Acanthamoeba and Naegleria were found in 45.5% of the samples, of which 41.6% were collected in the university hospital and 50% in the state hospital. Of all, 45.5% were positive for the genera Acanthamoeba and 3.8% for genera Naegleria. CONCLUSIONS: Potentially pathogenic free-living amoebas were seen in all sites of the two hospitals and Acanthamoeba was the most frequently isolated genera.
OBJECTIVE: To evaluate the occurrence of free-living amoebas of the genera Acanthamoeba and Naegleria is dust samples colleted in two hospitals. METHODS: One-hundred and thirty-two dust samples were collected in two hospitals in Brazil. Hospital collection sites were the following: intensive care unit, operation rooms, nursery, kitchen, emergency and infectious diseases isolation room. The isolation of the amoebas was performed in three culture media: non-nutrient agar inoculated with Escherichia coli, soy agar, and microculture in Giazzi-modified Pavlova's medium. The amoebas were identified according to morphological criteria. RESULTS: Amoebas of the genera Acanthamoeba and Naegleria were found in 45.5% of the samples, of which 41.6% were collected in the university hospital and 50% in the state hospital. Of all, 45.5% were positive for the genera Acanthamoeba and 3.8% for genera Naegleria. CONCLUSIONS: Potentially pathogenic free-living amoebas were seen in all sites of the two hospitals and Acanthamoeba was the most frequently isolated genera.
Authors: Natália Karla Bellini; Ana Letícia Moreira da Fonseca; María Reyes-Batlle; Jacob Lorenzo-Morales; Odete Rocha; Otavio Henrique Thiemann Journal: Pathogens Date: 2020-01-31
Authors: Natália Karla Bellini; Otavio Henrique Thiemann; María Reyes-Batlle; Jacob Lorenzo-Morales; Adriana Oliveira Costa Journal: Mem Inst Oswaldo Cruz Date: 2022-07-01 Impact factor: 2.747
Authors: Patrícia Albuquerque; André Moraes Nicola; Diogo Almeida Gomes Magnabosco; Lorena da Silveira Derengowski; Luana Soares Crisóstomo; Luciano Costa Gomes Xavier; Stefânia de Oliveira Frazão; Fernanda Guilhelmelli; Marco Antônio de Oliveira; Jhones do Nascimento Dias; Fabián Andrés Hurtado; Marcus de Melo Teixeira; Allan Jefferson Guimarães; Hugo Costa Paes; Eduardo Bagagli; Maria Sueli Soares Felipe; Arturo Casadevall; Ildinete Silva-Pereira Journal: PLoS Negl Trop Dis Date: 2019-10-07